dc.contributor |
Universitat de Barcelona |
dc.contributor.author |
Estil·les Altimiras, Elisabet |
dc.contributor.author |
Téllez i Besolí, Noèlia |
dc.contributor.author |
Escoriza, Jessica |
dc.contributor.author |
Montanya Mias, Eduard |
dc.date |
2019-06-07T17:03:43Z |
dc.date |
2019-06-07T17:03:43Z |
dc.date |
2012-10 |
dc.date |
2019-06-07T17:03:43Z |
dc.identifier.citation |
0963-6897 |
dc.identifier.citation |
622059 |
dc.identifier.uri |
http://hdl.handle.net/2445/134789 |
dc.format |
11 p. |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Cognizant Communication Corporation |
dc.relation |
Reproducció del document publicat a: https://doi.org/10.3727/096368912X638955 |
dc.relation |
Cell Transplantation, 2012, vol. 21, num. 10, p. 2119-2129 |
dc.relation |
https://doi.org/10.3727/096368912X638955 |
dc.rights |
cc-by-nc (c) Cognizant Communication Corporation, 2012 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
http://creativecommons.org/licenses/by-nc/3.0/es |
dc.subject |
Diabetis |
dc.subject |
Insulina |
dc.subject |
Biosíntesi |
dc.subject |
Illots de Langerhans |
dc.subject |
Regeneració (Biologia) |
dc.subject |
Cèl·lules B |
dc.subject |
Diabetes |
dc.subject |
Insulin |
dc.subject |
Biosynthesis |
dc.subject |
Islands of Langerhans |
dc.subject |
Regeneration (Biology) |
dc.subject |
B cells |
dc.title |
Increased beta cell replication, and beta cell mass regeneration in syngeneically transplanted rat islets overexpressing insulin-like growth factor-II |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |
dc.description.abstract |
Insulin-like growth factor II (IGF2) is a growth-promoting peptide that increases β-cell proliferation and survival. The aim of the study was to determine the effect of IGF2 overexpression on β-cell mass in transplanted islets. Islets infected with adenovirus encoding for IGF2 (Ad-IGF2 group), for luciferase (Ad-Luc control group), or with uninfected islets (control group) were syngeneically transplanted to streptozotocin-diabetic Lewis rats. Eight hundred islets, a minimal mass model to restore normoglycemia, or 500 islets, a clearly insufficient mass, were transplanted. Rats transplanted with 800 Ad-IGF2 islets showed a better metabolic evolution than control groups. As expected, rats transplanted with 500 Ad-IGF2 or control islets maintained similar hyperglycemia throughout the study, ensuring comparable metabolic conditions among both groups. β-Cell replication was higher in Ad-IGF2 group than in control group on days 3 [1.45% (IQR: 0.26) vs. 0.58% (IQR: 0.18), p = 0.006], 10 [1.58% (IQR: 1.40) vs. 0.90% (IQR: 0.61), p = 0.035], and 28 [1.35% (IQR: 0.35) vs. 0.64% (IQR: 0.28), p = 0.004] after transplantation. β-Cell mass was similarly reduced on day 3 after transplantation in Ad-IGF2 and control group [0.36 mg (IQR: 0.26) vs. 0.38 mg (IQR: 0.19)], it increased on day 10, and on day 28 it was higher in Ad-IGF2 than in control group [0.63 mg (IQR: 0.38) vs. 0.42 mg (IQR: 0.31), p = 0.008]. Apoptosis was similarly increased in Ad-IGF2 and control islets after transplantation. No differences in insulin secretion were found between Ad-IGF2 and uninfected control islets. In summary, IGF2 overexpression in transplanted islets increased β-cell replication, induced the regeneration of the transplanted β-cell mass, and had a beneficial effect on the metabolic outcome reducing the β-cell mass needed to achieve normoglycemia. |