Títol:
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An integrative study of protein-RNA condensates identifies scaffolding RNAs and reveals players in fragile X-associated tremor/ataxia syndrome
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Autor/a:
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Cid-Samper, Fernando; Gelabert-Baldrich, Mariona; Lang, Benjamin; Lorenzo Gotor, Maria de las Nieves; Ponti, Riccardo delli; Severijnen, Lies-Anne W.F.M.; Bolognesi, Benedetta; Gelpi, Ellen; Hukema, Renate K.; Botta Orfila, Teresa; Tartaglia, Gian Gaetano
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Abstract:
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Recent evidence indicates that specific RNAs promote the formation of ribonucleoprotein condensates by acting as scaffolds for RNA-binding proteins (RBPs). We systematically investigated RNA-RBP interaction networks to understand ribonucleoprotein assembly. We found that highly contacted RNAs are structured, have long UTRs, and contain nucleotide repeat expansions. Among the RNAs with such properties, we identified the FMR1 3' UTR that harbors CGG expansions implicated in fragile X-associated tremor/ataxia syndrome (FXTAS). We studied FMR1 binding partners in silico and in vitro and prioritized the splicing regulator TRA2A for further characterization. In a FXTAS cellular model, we validated the TRA2A-FMR1 interaction and investigated implications of its sequestration at both transcriptomic and post-transcriptomic levels. We found that TRA2A co-aggregates with FMR1 in a FXTAS mouse model and in post-mortem human samples. Our integrative study identifies key components of ribonucleoprotein aggregates, providing links to neurodegenerative disease and allowing the discovery of therapeutic targets. |
Abstract:
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The research leading to these results has been supported by the European Research Council (RIBOMYLOME_309545), Spanish Ministry of Economy and Competitiveness (BFU2014-55054-P and BFU2017-86970-P), and “Fundació La Marató de TV3” (PI043296). We acknowledge support of the Spanish Ministry of Economy and Competitiveness, ‘Centro de Excelencia Severo Ochoa 2013-2017’. We acknowledge the support of the CERCA Programme, Generalitat de Catalunya and Spanish Ministry for Science and Competitiveness (MINECO) to the EMBL partnership. |
Matèries:
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-Phase separation -Scaffolding RNA -CGG repeat expansion -FMR1 premutation -Fragile X-associated tremor/ataxia syndrome -FXTAS -RNA aggregates -RNA binding proteins -RBP -TRA2A splicing regulator -Neurodegeneration |
Drets:
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© 2018 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
Tipus de document:
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Article Article - Versió publicada |
Publicat per:
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Elsevier
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