(Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes

Home | About RECERCAT | Contact

Català | Castellano

All of RECERCAT

By Communities &
Collections By Defense Date By Authors By Titles By Subject

This Collection

By Defense Date By Authors By Titles By Subject

Statistics

View Statistics All RECERCAT

My RECERCAT

Other repositories directory

RECERCAT Home > Universitat Pompeu Fabra > Articles, congressos, llibres > View document

To access the full text documents, please follow this link: http://hdl.handle.net/10230/43001

dc.contributor.author	Neguembor, Maria Victoria
dc.contributor.author	Sebastián Pérez, Rubén
dc.contributor.author	Aulicino, Francesco, 1987-
dc.contributor.author	Gómez García, Pablo
dc.contributor.author	Cosma, Maria Pia, 1970-
dc.contributor.author	Lakadamyali, Melike
dc.date	2018
dc.identifier.citation	Neguembor MV, Sebastian-Perez R, Aulicino F, Gomez-Garcia PA, Cosma MP, Lakadamyali M. (Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes. Nucleic Acids Res. 2018; 46(5):e30. DOI 10.1093/nar/gkx1271
dc.identifier.citation	0305-1048
dc.identifier.citation	http://dx.doi.org/10.1093/nar/gkx1271
dc.identifier.uri	http://hdl.handle.net/10230/43001
dc.format	application/pdf
dc.language.iso	eng
dc.publisher	Oxford University Press
dc.relation	Nucleic Acids Res. 2018; 46(5):e30
dc.relation	info:eu-repo/grantAgreement/EC/H2020/686637
dc.relation	info:eu-repo/grantAgreement/ES/1PE/BFU2013–49867-EXP
dc.relation	info:eu-repo/grantAgreement/ES/1PE/BFU2014–54717-P
dc.relation	info:eu-repo/grantAgreement/ES/1PE/BFU2015–71984-ERC
dc.relation	info:eu-repo/grantAgreement/EC/FP7/608959
dc.relation	info:eu-repo/grantAgreement/EC/H2020/686637
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
dc.rights	http://creativecommons.org/licenses/by-nc/4.0/
dc.subject	Cell biology
dc.subject	Targeted gene modification
dc.title	(Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion
dc.description.abstract	CRISPR/dCas9-based labeling has allowed direct visualization of genomic regions in living cells. However, poor labeling efficiency and signal-to-background ratio have limited its application to visualize genome organization using super-resolution microscopy. We developed (Po)STAC (Polycistronic SunTAg modified CRISPR) by combining CRISPR/dCas9 with SunTag labeling and polycistronic vectors. (Po)STAC enhances both labeling efficiency and fluorescence signal detected from labeled loci enabling live cell imaging as well as super-resolution fixed-cell imaging of multiple genes with high spatiotemporal resolution.
dc.description.abstract	European Union’s Horizon 2020 Research and Innovation Programme [CellViewer No 686637 to M.L., M.P.C.]; Ministerio de Economia y Competitividad [BFU2013–49867-EXP to M.L., M.P.C.]; Fundació Cellex Barcelona (to M.L); European Union Seventh Framework Programme under the European Research Council Grants [337191-MOTORS to M.L.]; ‘Severo Ochoa’ Programme for Centres of Excellence in R&D [SEV-2015- 0522 to M.L.]; Ministerio de Economia y Competitividad and FEDER Funds [BFU2014–54717-P, BFU2015–71984-ERC to M.P.C.]; AGAUR Grant [2014 SGR1137 to M.P.C.]; Spanish Ministry of Economy and Competitiveness (to M.P.C.); Centro de Excelencia Severo Ochoa [2013–2017 to M.P.C.]; CERCA Programme/Generalitat de Catalunya (to M.P.C); Ministerio de Ciencia e Innovacion FPI (to F.A.); People Program (Marie Curie Actions) FP7/2007–2013 under REA grant [608959 to M.V.N.]. Funding for open access charge: European Union’s Horizon 2020 Research and Innovation Programme [CellViewer No 686637].

Show simple item record

All of RECERCAT

This Collection

Statistics

My RECERCAT

Related documents

Other documents of the same author