dc.contributor.author |
Fernández Ulibarri, Inés |
dc.contributor.author |
Vilella, Montserrat |
dc.contributor.author |
Lázaro Diéguez, Francisco |
dc.contributor.author |
Sarri Plans, Elisabet |
dc.contributor.author |
Martínez, Susana E. |
dc.contributor.author |
Jiménez, Nuria |
dc.contributor.author |
Claro Izaguirre, Enrique |
dc.contributor.author |
Mérida, Isabel |
dc.contributor.author |
Burger, Koert N. J. |
dc.contributor.author |
Egea, Gustavo |
dc.date |
2007 |
dc.identifier |
https://ddd.uab.cat/record/143838 |
dc.identifier |
urn:10.1091/mbc.E07-04-0334 |
dc.identifier |
urn:17567948 |
dc.identifier |
urn:pmid:17567948 |
dc.identifier |
urn:recercauab:ARE-44204 |
dc.identifier |
urn:articleid:19394586v18n9p3250 |
dc.identifier |
urn:scopus_id:34548496498 |
dc.identifier |
urn:wos_id:000249162200003 |
dc.identifier |
urn:oai:egreta.uab.cat:publications/0005b1cd-1b61-4f8c-9917-a9c98e4b400d |
dc.identifier |
urn:pmc-uid:1951743 |
dc.identifier |
urn:pmcid:PMC1951743 |
dc.identifier |
urn:oai:pubmedcentral.nih.gov:1951743 |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
|
dc.relation |
Ministerio de Educación y Ciencia BFU2006/0086 |
dc.relation |
European Commission RTN2002/00259 |
dc.relation |
Molecular biology of the cell ; Vol. 18, Num. 9 (2007), p. 3250-3263 |
dc.rights |
open access |
dc.rights |
Tots els drets reservats. |
dc.rights |
https://rightsstatements.org/vocab/InC/1.0/ |
dc.title |
Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway |
dc.type |
Article |
dc.description.abstract |
Diacylglycerol is necessary for trans-Golgi network (TGN) to cell surface transport, but its functional relevance in the early secretory pathway is unclear. Although depletion of diacylglycerol did not affect ER-to-Golgi transport, it led to a redistribution of the KDEL receptor to the Golgi, indicating that Golgi-to-ER transport was perturbed. Electron microscopy revealed an accumulation of COPI-coated membrane profiles close to the Golgi cisternae. Electron tomography showed that the majority of these membrane profiles originate from coated buds, indicating a block in membrane fission. Under these conditions the Golgi-associated pool of ARFGAP1 was reduced, but there was no effect on the binding of coatomer or the membrane fission protein CtBP3/BARS to the Golgi. The addition of 1,2-dioctanoyl-sn-glycerol or the diacylglycerol analogue phorbol 12,13-dibutyrate reversed the effects of endogenous diacylglycerol depletion. Our findings implicate diacylglycerol in the retrograde transport of proteins from Golgi to the ER and suggest that it plays a critical role at a late stage of COPI vesicle formation. |