dc.contributor.author |
Balseiro, Ana |
dc.contributor.author |
Altuzarra, Raúl |
dc.contributor.author |
Vidal Barba, Enric |
dc.contributor.author |
Moll Sánchez, Xavier |
dc.contributor.author |
Espada, Yvonne |
dc.contributor.author |
Sevilla, Iker A. |
dc.contributor.author |
Domingo, Mariano |
dc.contributor.author |
Garrido, Joseba M. |
dc.contributor.author |
Juste, Ramón A. |
dc.contributor.author |
Prieto, Miguel |
dc.contributor.author |
Pérez de Val, Bernat |
dc.date |
2017 |
dc.identifier |
https://ddd.uab.cat/record/201311 |
dc.identifier |
urn:10.1371/journal.pone.0180546 |
dc.identifier |
urn:oai:ddd.uab.cat:201311 |
dc.identifier |
urn:pmid:28678885 |
dc.identifier |
urn:scopus_id:85021809154 |
dc.identifier |
urn:recercauab:ARE-85895 |
dc.identifier |
urn:articleid:19326203v12n7e0180546 |
dc.identifier |
urn:wos_id:000405272200072 |
dc.identifier |
urn:altmetric_id:21539077 |
dc.identifier |
urn:oai:egreta.uab.cat:publications/27e758e9-b8a3-4091-8109-6e4d6eb1a585 |
dc.identifier |
urn:pmc-uid:5498051 |
dc.identifier |
urn:pmcid:PMC5498051 |
dc.identifier |
urn:oai:pubmedcentral.nih.gov:5498051 |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
|
dc.relation |
PloS one ; Vol. 12 Núm. 7 (july 2017), p. e0180546 |
dc.rights |
open access |
dc.rights |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
dc.rights |
https://creativecommons.org/licenses/by/4.0/ |
dc.subject |
Hydrogen-Ion Concentration |
dc.title |
Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep |
dc.type |
Article |
dc.description.abstract |
Altres ajuts: INIA/RTA2014-0002-C02-01 |
dc.description.abstract |
Animal tuberculosis (TB) is a complex animal health problem that causes disruption to trade and significant economic losses. TB involves a multi-host system where sheep, traditionally considered a rare host of this infection, have been recently included. The aims of this study were to develop an experimental TB infection model in sheep with a Mycobacterium caprae field strain isolated from a tuberculous diseased ewe, and to use this to evaluate the safety and efficacy of two vaccines against TB in sheep, the live-attenuated M. bovis BCG vaccine (Danish strain) and a heat-inactivated M. bovis (HIMB) vaccine. Methods: Eighteen 2 month-old lambs were experimentally challenged with M. caprae by the endotracheal route (1.5 × 10 CFU). They were separated per treatment group into parenterally vaccinated with a live BCG Danish strain vaccine (n = 6), orally vaccinated with a suspension of HIMB (n = 6) and unvaccinated controls (n = 6). Clinical, immunological, pathological and bacteriological parameters of infection were measured. Results: All lambs were successfully infected and developed gross TB lesions in the respiratory system. The BCG vaccine conferred considerable protection against experimental TB in lambs, as measured by a reduction of the gross lesion volumes and bacterial load. However, HIMB vaccinated animals did not show protection. Conclusions: This study proposes a reliable new experimental model for a better understanding of tuberculosis in sheep. BCG vaccination offers an effective prospect for controlling the disease. Moreover alternative doses and/or routes of administration should be considered to evaluate the efficacy of the HIMB vaccine candidate. |