dc.contributor.author |
García, Juan Ignacio |
dc.contributor.author |
Kelley, Holden V. |
dc.contributor.author |
Meléndez, Johanna |
dc.contributor.author |
Álvarez de León, Rosa Alejandra |
dc.contributor.author |
Castillo, Alejandra |
dc.contributor.author |
Sidiki, Sabeen |
dc.contributor.author |
Yusoof, Kizil A. |
dc.contributor.author |
Nunes, Elizabete |
dc.contributor.author |
López Téllez, César |
dc.contributor.author |
Mejía Villatoro, Carlos Rodolfo |
dc.contributor.author |
Ikeda, Janet |
dc.contributor.author |
García-Basteiro, Alberto L.. |
dc.contributor.author |
Wang, Shu Hua |
dc.contributor.author |
Torrelles, Jordi B. |
dc.date |
2019 |
dc.identifier |
https://ddd.uab.cat/record/223311 |
dc.identifier |
urn:10.1038/s41598-019-54537-9 |
dc.identifier |
urn:oai:ddd.uab.cat:223311 |
dc.identifier |
urn:scopus_id:85075802088 |
dc.identifier |
urn:articleid:20452322v9n1p18012 |
dc.identifier |
urn:pmid:31784649 |
dc.identifier |
urn:pmc-uid:6884436 |
dc.identifier |
urn:pmcid:PMC6884436 |
dc.identifier |
urn:oai:pubmedcentral.nih.gov:6884436 |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
|
dc.relation |
Scientific reports ; Vol. 9 Núm. 1 (january 2019), p. 18012 |
dc.rights |
open access |
dc.rights |
Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. |
dc.rights |
https://creativecommons.org/licenses/by/4.0/ |
dc.title |
Improved Alere Determine Lipoarabinomannan Antigen Detection Test for the Diagnosis of Human and Bovine Tuberculosis by Manipulating Urine and Milk |
dc.type |
Article |
dc.description.abstract |
Tuberculosis (TB) disease still kills 1-person every 21-seconds. Few TB diagnostic tests are considered truly appropriate for point of care settings. The WHO-endorsed immunodiagnostic Alere Determine Lipoarabinomannan Ag-test (LAM-test) detects Mycobacterium tuberculosis complex LAM in urine, and its use is recommended for TB diagnosis among HIV co-infected individuals with low CD4 T-cell counts. Here we found that a simple 15-minute enzymatic treatment at room temperature of LAM-spiked urine with α-mannosidase (for human TB), and LAM-spiked milk with combined lactase and caseinase (for bovine TB), enhanced 10-fold the detection levels of the LAM-test and thus, improved the detection of LAM by the LAM-test in urine and milk that otherwise could be missed in the field. Future separate clinical research studies specifically designed to address the potential of these findings are required. |