The magnitude of inter-limb asymmetries has been reported in several studies in different team sports but only some of them have analysed their effects on sports performance. The main purpose of this cross-sectional study was to determine inter-limb asymmetries and examine their relationship with different physical performance tests in rink hockey players. Nineteen rink hockey players (age: 23.37 ± 4.82 years; body height: 1.75 ± 0.06 cm; body mass: 73.16 ± 9.87 kg) performed a mid-season battery of fitness tests consisting of 30 m sprint, countermovement jump (CMJ), and half back squat. Inter-limb asymmetries were assessed using the single leg vertical countermovement jump test (SLCJ-V), single leg horizontal countermovement jump test (SLCJ-H), and 180° change of direction test (COD). Results show a significant relationship between asymmetry in the SLCJ-H test, 30 m sprint (r = 0.63, p < 0.01) and CMJ performances (r = −0.52, p < 0.05). Additionally, asymmetry in the SLCJ-V showed a significant relationship with CMJ performance (r = −0.46, p < 0.05). These findings suggest that training programs should aim to reduce the inter-limb asymmetries in rink hockey players to improve their performance. Practitioners and coaches can use this information to assess the potential impact of asymmetries on physical performance in rink hockey players.