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Title:	miRNA let-7e modulates the wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation
Author:	Viñas Muñoz, José Luis; Ventayol Espinazo, Marina; Brüne, Bernhard, 1957-; Jung, Michaela; Sola Martínez, Anna; Pi i Siqués, Felip; Mastora, Chrysoula; Hotter Corripio, Georgina
Notes:	This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3β(P)) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3β(P) and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production.
Subject(s):	-Cèl·lules mare embrionàries -Micro RNAs -Diferenciació cel·lular -Embryonic stem cells -MicroRNAs -Cell diferentiation
Rights:	cc-by (c) Viñas Muñoz, José Luis et al., 2013 http://creativecommons.org/licenses/by/3.0/es
Document type:	Article Article - Published version
Published by:	Public Library of Science (PLoS)
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