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Title:	Myogenin protein stability is decreased by BMP-2 through a mechanism implicating Id1
Author:	Viñals Canals, Francesc; Ventura Pujol, Francesc
Other authors:	Universitat de Barcelona
Abstract:	Bone morphogenetic protein-2 (BMP-2) induces a switch in differentiation of mesenchymal cells from the myogenic to the osteogenic lineage. Here we describe that in C2C12 cells, BMP-2 decreases myogenin expression induced by des-(1,3) insulin-like growth factor-1 (des-(1,3)IGF-1) or ectopically expressed from a constitutive promoter, even in conditions where myogenin mRNA levels were unaffected. Addition of BMP-2 decreases myogenin protein half-life to 50%, whereas proteasome inhibitors abolish these effects. Forced expression of Id1, either by transient transfection or under the control of an inducible system, causes degradation of myogenin in the absence of BMP-2. In contrast, E47 overexpression blocks the inhibitory effect of BMP-2 on myogenin levels. Finally, expression of E47 in 293 cells stabilizes myogenin, an effect that is dependent on the heterodimerization mediated by their helix-loop-helix. Our findings indicate that induction of Id1 not only blocks transcriptional activity but also induces myogenin degradation by blocking formation of myogenin-E47 protein complexes.
Subject(s):	-Proteïnes -Factors de transcripció -Factors de creixement -Transcripció genètica -Miogènesi -Proteins -Transcription factors -Growth factors -Genetic transcription -Myogenesis
Rights:	(c) American Society for Biochemistry and Molecular Biology, 2004
Document type:	Article Article - Published version
Published by:	American Society for Biochemistry and Molecular Biology
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