Título:
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Distinct Trends of DNA Methylation Patterning in the Innate and Adaptive Immune Systems
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Autor/a:
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Schuyler, Ronald P.; Merkel, Angelika; Raineri, Emanuele; Gut, Ivo Glynne; Raineri; Heath, Simon
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Abstract:
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DNA methylation and the localization and post-translational modification of nucleosomes are interdependent factors that contribute to the generation of distinct phenotypes from genetically identical cells. With 112 whole-genome bisulfite sequencing datasets from the BLUEPRINT Epigenome Project, we analyzed the global development of DNA methylation patterns during lineage commitment and maturation of a range of immune system effector cells and the cancers that arise from them. We show clear trends in methylation patterns that are distinct in the innate and adaptive arms of the human immune system, both globally and in relation to consistently positioned nucleosomes. Most notable are a progressive loss of methylation in developing lymphocytes and the consistent occurrence of non-CG methylation in specific cell types. Cancer samples from the two lineages are further polarized, suggesting the involvement of distinct lineage-specific epigenetic mechanisms. We anticipate broad utility for this resource as a basis for further comparative epigenetic analyses. |
Abstract:
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K.D. is funded as a HSST trainee by NHS Health Education England. M.F. is supported by the BHF Cambridge Centre of Excellence (RE/13/6/30180). Research in the W.H.O. laboratory is supported by EU-FP7 project BLUEPRINT (282510) and by program grants from the NIHR (http://www.nihr.ac.uk) and the British Heart Foundation under numbers RP-PG-0310-1002 and RG/09/12/28096 (https://www.bhf.org.uk). The laboratory receives funding from the NHS Blood and Transplant for facilities. L.C., A.D., E.L., and P.F. also acknowledge support from the European Molecular Biology Laboratory. |
Materia(s):
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-DNA methylation -Epigenetics -Whole-genome bisulfite sequencing -Hematopoiesis -CTCF -Nucleosomes -BLUEPRINT |
Derechos:
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https://creativecommons.org/licenses/by/4.0/
© Elsevier http://dx.doi.org/10.1016/j.celrep.2016.10.054. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited |
Tipo de documento:
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Artículo Artículo - Versión publicada |
Editor:
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Elsevier
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Compartir:
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