Títol:
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Genome-wide analysis of differential transcriptional and epigenetic variability across human immune cell types
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Autor/a:
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Ecker, Simone; Merkel, Angelika; BLUEPRINT Consortium; Paul, Dirk S.
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Abstract:
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Background: A healthy immune system requires immune cells that adapt rapidly to environmental challenges. This phenotypic plasticity can be mediated by transcriptional and epigenetic variability. Results: We apply a novel analytical approach to measure and compare transcriptional and epigenetic variability genome-wide across CD14+CD16− monocytes, CD66b+CD16+ neutrophils, and CD4+CD45RA+ naïve T cells from the same 125 healthy individuals. We discover substantially increased variability in neutrophils compared to monocytes and T cells. In neutrophils, genes with hypervariable expression are found to be implicated in key immune pathways and are associated with cellular properties and environmental exposure. We also observe increased sex-specific gene expression differences in neutrophils. Neutrophil-specific DNA methylation hypervariable sites are enriched at dynamic chromatin regions and active enhancers. Conclusions: Our data highlight the importance of transcriptional and epigenetic variability for the key role of neutrophils as the first responders to inflammatory stimuli. We provide a resource to enable further functional studies into the plasticity of immune cells, which can be accessed from: http://blueprint-dev.bioinfo.cnio.es/WP10/hypervariability. |
Abstract:
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This work is predominantly funded by the EU-FP7 Project BLUEPRINT (HEALTH-F5-2011-282510). |
Matèries:
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-Differential variability -Phenotypic plasticity -Heterogeneity -Immune cells -Monocytes -Neutrophils -T cells -Gene expression -DNA methylation |
Drets:
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© Ecker E, et al. 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
http://creativecommons.org/licenses/by/4.0/ |
Tipus de document:
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Article Article - Versió publicada |
Publicat per:
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BioMed Central
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