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Title:
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Studies on the biocontrol mechanisms of Pseudomonas graminis strain CPA-7 against food-borne pathogens in vitro and on fresh-cut melon
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Author:
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Collazo Cordero, Cyrelys; Abadias i Sero, Mª Isabel; Aguiló-Aguayo, Ingrid; Alegre Vilas, Isabel; Chenoll, Empar; Viñas Almenar, Inmaculada
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Notes:
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The present study was aimed at gaining insight into the mode of action of the antagonistic bacteria Pseudomonas graminis CPA-7, which has been previously identified as an effective biocontrol agent against Listeria monocytogenes, Salmonella enterica and Escherichia coli O157:H7 on fresh-cut fruit. In vitro experiments did not reveal any antimicrobial or proteolytic activity on solid media or any biosurfactant activity on hydrophobic surfaces. Metabolites produced by CPA-7 in two different culture media and on 'Galia' melon were unable to inhibit L. monocytogenes populations on 'Galia' melon plugs at 25 °C or 5 °C. In contrast, at 25 °C the population of this pathogen on 'Galia' plugs was reduced by 2.1 and 3.3 log-units when co-inoculated with the antagonist in water, after 24 and 48 h, respectively. CPA-7 did not form biofilms after 72 h at 25 °C (OD = 0.03) or at 30 °C (OD = 0.01) on polystyrene plates and the production of alginate was close to the negative control. Studies of nutritional profiles showed high overlap (NOI > 0.9) between CPA-7 and E. coli O157:H7 regarding the use of carboxylic acids. This functional group could also contain putative targets for competiveness between CPA-7 and S. enterica, although overlapping was not restrictive enough (NOI = 0.83).
The authors are grateful to the Catalan departmental government for the Phd. FI-DGR-2015-0004 grant (Cyrelys Collazo) and to the Spanish central government for the FDPI-2013-1583 grant (Dr. Ingrid Aguiló-Aguayo). |
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Subject(s):
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-Biological control
-Action mechanism
-Antagonist
-Fresh-cut melon |
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Rights:
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cc-by-nc-nd (c) Elsevier, 2017
http://creativecommons.org/licenses/by-nc-nd/4.0/
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Document type:
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Article
Article - Accepted version |
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Published by:
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Elsevier
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