dc.contributor.author |
Collazo Cordero, Cyrelys |
dc.contributor.author |
Abadias i Sero, Mª Isabel |
dc.contributor.author |
Aguiló-Aguayo, Ingrid |
dc.contributor.author |
Alegre Vilas, Isabel |
dc.contributor.author |
Chenoll, Empar |
dc.contributor.author |
Viñas Almenar, Inmaculada |
dc.date |
2018-10-31T12:01:47Z |
dc.date |
2018-10-31T12:01:47Z |
dc.date |
2017 |
dc.identifier |
0023-6438 |
dc.identifier |
http://hdl.handle.net/10459.1/65019 |
dc.identifier |
https://doi.org/10.1016/j.lwt.2017.02.029 |
dc.identifier.uri |
http://hdl.handle.net/10459.1/65019 |
dc.description |
The present study was aimed at gaining insight into the mode of action of the antagonistic bacteria Pseudomonas graminis CPA-7, which has been previously identified as an effective biocontrol agent against Listeria monocytogenes, Salmonella enterica and Escherichia coli O157:H7 on fresh-cut fruit. In vitro experiments did not reveal any antimicrobial or proteolytic activity on solid media or any biosurfactant activity on hydrophobic surfaces. Metabolites produced by CPA-7 in two different culture media and on 'Galia' melon were unable to inhibit L. monocytogenes populations on 'Galia' melon plugs at 25 °C or 5 °C. In contrast, at 25 °C the population of this pathogen on 'Galia' plugs was reduced by 2.1 and 3.3 log-units when co-inoculated with the antagonist in water, after 24 and 48 h, respectively. CPA-7 did not form biofilms after 72 h at 25 °C (OD = 0.03) or at 30 °C (OD = 0.01) on polystyrene plates and the production of alginate was close to the negative control. Studies of nutritional profiles showed high overlap (NOI > 0.9) between CPA-7 and E. coli O157:H7 regarding the use of carboxylic acids. This functional group could also contain putative targets for competiveness between CPA-7 and S. enterica, although overlapping was not restrictive enough (NOI = 0.83). |
dc.description |
The authors are grateful to the Catalan departmental government for the Phd. FI-DGR-2015-0004 grant (Cyrelys Collazo) and to the Spanish central government for the FDPI-2013-1583 grant (Dr. Ingrid Aguiló-Aguayo). |
dc.language |
eng |
dc.publisher |
Elsevier |
dc.relation |
Versió postprint del document publicat a https://doi.org/10.1016/j.lwt.2017.02.029 |
dc.relation |
LWT - Food Science and Technology, 2017, vol. 85, Part B, p. 301-308 |
dc.rights |
cc-by-nc-nd (c) Elsevier, 2017 |
dc.rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Biological control |
dc.subject |
Action mechanism |
dc.subject |
Antagonist |
dc.subject |
Fresh-cut melon |
dc.title |
Studies on the biocontrol mechanisms of Pseudomonas graminis strain CPA-7 against food-borne pathogens in vitro and on fresh-cut melon |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/acceptedVersion |