The use of e-portfolio becomes a standard tool when it comes to learning and student's assessment. This is due to the teachers need for enhancing their students’ autonomy. The use of e-portfolio helps students to focus on their own learning process. Lectures should not be limited only to classes, but must foster active learning, and in this regard, the introduction of the e-portfolio is an excellent tool because it stimulates collaborative and cooperative work among students and, in turn, it encourages feedback by the teacher. To apply active methodologies during 2014-15, the e-portfolio was introduced in the course of Linear Algebra. To prepare the work of the e-portfolio the teacher had to define precisely the objectives that must be achieved by the students, and has had to plan in an understandable manner the tasks that the students can work independently outside the classroom. For the realization of the e-portfolio have been used different platforms. Each quarter of the students worked on a different platform, through AteneaLabs that provided templates so that each student make their own e-portfolio, as well as it provide all necessary manuals. The platforms used were: Mahara, Exabis, WordPress and Google Sites. Formative assessment of the e-portfolio had been made from different rubrics defined in the course syllabus and known by students since the beginning of the course.