Title:
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Three-dimensional genomic structure and cohesin occupancy correlate with transcriptional activity during spermatogenesis
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Author:
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Vara González, Covadonga; Paytuví-Gallart, Andreu; Cuartero, Yasmina; Le Dily, François; García, Francisca; Salvà Castro, Judith; Gomez Hernández, Laura; Julià, Eva; Moutinho, Catia; Aiese Cigliano, Riccardo; Sanseverino, Walter; Fornas, Òscar; Martín Pendas, Alberto; Heyn, Holger; Waters, Paul D.; Martí Renom, Marc A.; Ruiz-Herrera Moreno, Aurora
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Abstract:
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Altres ajuts: Agaur SGR468 to M.A.M-R. A.P.-G. is supported by a 'Doctorats Industrials' predoctoral fellowship (AGAUR). H.H. is a Miguel Servet (CP14/00229) researcher funded by the Spanish Institute of Health Carlos III (ISCIII) |
Abstract:
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Mammalian gametogenesis involves dramatic and tightly regulated chromatin remodeling, whose regulatory pathways remain largely unexplored. Here, we generate a comprehensive high-resolution structural and functional atlas of mouse spermatogenesis by combining in situ chromosome conformation capture sequencing (Hi-C), RNA sequencing (RNA-seq), and chromatin immunoprecipitation sequencing (ChIP-seq) of CCCTC-binding factor (CTCF) and meiotic cohesins, coupled with confocal and super-resolution microscopy. Spermatogonia presents well-defined compartment patterns and topological domains. However, chromosome occupancy and compartmentalization are highly re-arranged during prophase I, with cohesins bound to active promoters in DNA loops out of the chromosomal axes. Compartment patterns re-emerge in round spermatids, where cohesin occupancy correlates with transcriptional activity of key developmental genes. The compact sperm genome contains compartments with actively transcribed genes but no fine-scale topological domains, concomitant with the presence of protamines. Overall, we demonstrate how genome-wide cohesin occupancy and transcriptional activity is associated with three-dimensional (3D) remodeling during spermatogenesis, ultimately reprogramming the genome for the next generation. |
Rights:
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open access
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https://creativecommons.org/licenses/by/4.0/ |
Document type:
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Article |
Published by:
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Uri:
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https://ddd.uab.cat/record/223523
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