|
Título:
|
The Forkhead Transcription Factor Hcm1 Promotes Mitochondrial Biogenesis and Stress Resistance in Yeast
|
|
Autor/a:
|
Rodríguez Colman, Maria José; Reverter Branchat, Gemma; Sorolla Bardají, Maria Alba; Tamarit Sumalla, Jordi; Ros Salvador, Joaquim; Cabiscol Català, Elisa
|
|
Notas:
|
In Saccharomyces cerevisiae, the forkhead transcription factor
Hcm1 is involved in chromosome segregation, spindle pole
dynamics, and budding. We found that Hcm1 interacts with the
histone deacetylase Sir2 and shifts from cytoplasm to the
nucleus in the G1/S phase or in response to oxidative stress stimuli.
The nuclear localization of Hcm1 depends on the activity of
Sir2 as revealed by activators and inhibitors of the sirtuins and
the sir2 mutant. Hcm1-overexpressing cells display more
mitochondria that can be attributed to increased amounts of
Abf2, a protein involved in mitochondrial biogenesis. These
cells also show higher rates of oxygen consumption and
improved resistance to oxidative stress that would be explained
by increased catalase and Sod2 activities and molecular chaperones
such as Hsp26, Hsp30, and members of Hsp70 family.
Microarray analyses also reveal increased expression of genes
involved in mitochondrial energy pathways and those allowing
the transition from the exponential to the stationary phase.
Taken together, these results describe a new and relevant role of
Hcm1 for mitochondrial functions, suggesting that this transcription
factor would participate in the adaptation of cells from
fermentative to respiratory metabolism.
This work has been supported by grants BFU2007-66249 and CSD2007-00020 Consolider Ingenio 2010 from the Ministerio de Ciencia e Innovación (Spain) and SGR2009-00196 from the Generalitat de Catalunya. |
|
Derechos:
|
(c) The American Society for Biochemistry and Molecular Biology, 2010
|
|
Tipo de documento:
|
article
publishedVersion |
|
Editor:
|
American Society for Biochemistry and Molecular Biology
|
|
Compartir:
|
|
