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Título:
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Transgenic wheat plants expressing an oat arginine decarboxylase cDNA exhibit increases in polyamine content in vegetative tissue and seeds
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Autor/a:
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Bassie Rene, Ludovic; Zhu, Changfu; Capell Capell, Teresa; Romagosa Clariana, Ignacio; Christou, Paul
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Notas:
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We generated and characterized a trans-
genic wheat (
Triticum aestivum
L.) population
expressing constitutively an oat
arginine decarboxylase
(
ADC
) cDNA. Transgenic wheat plants expressing the
transgene had a significant increase in ADC activity
with no concomitant changes in ODC activity. We
measured 2-fold increases in putrescine, spermidine and
spermine content in leaves of transgenic plants com-
pared to wild-type. These plants also exhibited
significant increases in the three polyamines in seeds
(7-fold putrescine, 2.5-fold spermidine and 1.8-fold
spermine). This significant polyamine accumulation
was heritable in subsequent generations. In order to
characterize molecularly this population we cloned a
partial
TaADC
cDNA. This sequence was used as a
probe to ascertain the presence of two distinct ADC
transcripts in wheat. Further DNA analyses confirmed
the presence of at least two distinct
ADC
genes in the
wheat genome. Our results demonstrate that polyamine
levels can be altered in transgenic wheat plants resulting
in phenotypically normal and fertile plants. This
transgenic wheat population will serve as useful germ-
plasm to embark on further in depth studies to ascertain
the role of polyamines in abiotic stress responses.
This work was supported by Grant AGL2004-00444 from the Ministerio de Ciencia y Tecnologia (Spain) to T.C. and ICA3- CT2002-10026 (MABDE) from EU to I.R. L.B. is a Juan de la Cierva researcher; T.C. is a tenure-track scientist of the Ramo ́ n y Cajal Programme; P.C. is an ICREA researcher, all at the UdL |
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Materia(s):
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-Arginine decarboxylase
-Polyamines
-Transgene expression
-Transgenic wheat
-Wheat ADC genes |
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Derechos:
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(c) Springer, 2008
info:eu-repo/semantics/restrictedAccess |
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Tipo de documento:
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article
publishedVersion |
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Editor:
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Springer
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Compartir:
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