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Título:
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Joint QTL mapping and gene expression analysis identify positional candidate genes influencing pork quality traits
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Autor/a:
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González Prendes, Rayner; Quintanilla Aguado, Raquel; Cánovas, Angela; Manunza, Arianna; Figueiredo Cardoso, Tainã; Jordana i Vidal, Jordi; Noguera Jiménez, José Luis; Pena i Subirà, Ramona Natacha; Amills i Eras, Marcel
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Notas:
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Meat quality traits have an increasing importance in the pig industry because of their strong impact on consumer acceptance. Herewith, we have combined phenotypic and microarray expression data to map loci with potential effects on five meat quality traits recorded in the longissimus dorsi (LD) and gluteus medius (GM) muscles of 350 Duroc pigs, i.e. pH at 24 hours post-mortem (pH24), electric conductivity (CE) and muscle redness (a*), lightness (L*) and yellowness (b*). We have found significant genome-wide associations for CE of LD on SSC4 (~104 Mb), SSC5 (~15 Mb) and SSC13 (~137 Mb), while several additional regions were significantly associated with meat quality traits at the chromosome-wide level. There was a low positional concordance between the associations found for LD and GM traits, a feature that reflects the existence of differences in the genetic determinism of meat quality phenotypes in these two muscles. The performance of an eQTL search for SNPs mapping to the regions associated with meat quality traits demonstrated that the GM a* SSC3 and pH24 SSC17 QTL display positional concordance with cis-eQTL regulating the expression of several genes with a potential role on muscle metabolism.
The authors are indebted to Selección Batallé S.A. for providing the animal material. We gratefully acknowledge to J. Reixach (Selección Batallé), I. Díaz (IRTA) and J. Soler (IRTA) for their collaboration in the experimental protocols. Thanks to Anna Mercadé and Anna Castelló for their technical support. This research was partially funded with projects AGL2013-48742-C2-1-R and AGL2013-48742-C2-2-R, awarded by the Spanish Ministry of Economy and Competitiveness (MINECO). We also acknowledge the support of the Spanish Ministry of Economy and Competitiveness for the Center of Excellence Severo Ochoa 2016–2019 (SEV-2015-0533) grant awarded to the Center for Research in Agricultural Genomics. Rayner Gonzalez-Prendes was funded by a FPU Ph.D. grant from the Spanish Ministerio de Educación (FPU12/00860). Thanks also to the CERCA Programme of the Generalitat de Catalunya. |
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Materia(s):
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-Animal breeding
-Gene expression
-Genome-wide association studies |
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Derechos:
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cc-by (c) González Prendes, Rayner. et al., 2017
http://creativecommons.org/licenses/by/4.0/
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Tipo de documento:
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article
publishedVersion |
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Editor:
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Nature
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Compartir:
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