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Title:
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Influence of temperature, water activity and incubation time on fungal growth and production of ochratoxin A and zearalenone by toxigenic Aspergillus tubingensis and Fusarium incarnatum isolates in sorghum seeds
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Author:
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Lahouar, Amani; Marín Sillué, Sònia; Crespo Sempere, Ana; Saïd, Salem; Sanchís Almenar, Vicente
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Notes:
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The major objective of this study was to describe the effect of water activity and temperature on radial growth
and production of ochratoxin A (OTA) and zearalenone (ZEA) on sorghum grains of three Aspergillus tubingensis
and three Fusarium incarnatum isolates. The water activity range was 0.91–0.99 aw for F. incarnatum isolates and
0.88–0.99 aw for A. tubingensis isolates. Temperatures of incubation were 15, 25 and 37 °C for both species. Mycotoxin
production was determined after 7, 14, 21 and 28 days depending on the growth rate of the six isolates.
Maximum growth rates (mm/day) were observed at 37 °C and 0.99 aw for A. tubingensis isolates and at 0.99 aw
and 25 °C for F. incarnatum isolates. A. tubingensis was able to grow at 15 °C only at the highest aw levels (0.97
and 0.99 aw). However, at this temperature F. incarnatum grew at 0.94 aw. Optimum ochratoxin A production
was observed at 0.97 aw × 37 °Cwhereas optimal conditions for ZEA production varied fromone isolate to another.
Moreover, isolates of F. incarnatum from Tunisia do not require high aw and temperature levels to yield maximum
levels of ZEA. In general, our results showed that there is no correlation between the growth and
production of ZEA in the case of F. incarnatum. This is the first study on the water activity and temperature effect
on growth rate and ZEA production of F. incarnatum. Our results show that sorghum grains not only support
growth but also OTA and ZEA production by A. tubingensis and F. incarnatum, respectively.
The authors are grateful to the European Union (MYCORED KBBE-2007-2-5-05 project) and Tunisian Government (U12E503) for the financial support. |
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Subject(s):
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-Aspergillus tubingensis
-Fusarium incarnatum
-Ochratoxin A
-Zearalenone |
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Rights:
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(c) Elsevier B.V., 2016
info:eu-repo/semantics/restrictedAccess |
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Document type:
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article
publishedVersion |
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Published by:
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Elsevier
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