Autor/a:
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Soler, Cassandra; Goossens, Tim; Bermejo, Alvaro; Migura-García, Lourdes; Cusco, Anna; Francino, Olga; Fraile Sauce, Lorenzo José
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Notas:
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Antimicrobials have been used in a prophylactic way to decrease the incidence of digestive
disorders during the piglet post-weaning period. Nowadays, it is urgent to reduce their consumption
in livestock to address the problem of antimicrobial resistance. In this study, the
effect of a product on piglet microbiota has been investigated as an alternative to antimicrobials.
Three groups of ten post-weaning pigs were sampled at 0, 15 and 30 days one week
post-weaning; the control, antibiotic and feed additive group received a standard post-weaning
diet without antibiotics or additives, the same diet as the control group but with amoxicillin
and colistin sulphate and the same diet as the control group but with a feed additive
(Sanacore-EN, Nutriad International N.V.), respectively. The total DNA extracted from faeces
was used to amplify the 16S RNA gene for massive sequencing under manufacturer's
conditions. Sequencing data was quality filtered and analyzed using QIIME software and
suitable statistical methods. In general terms, age modifies significantly the microbiota of
the piglets. Thus, the oldest the animal, the highest bacterial diversity observed for the control
and the feed additive groups. However, this diversity was very similar in the antibiotic
group throughout the trial. Interestingly, a clear increase in abundance of Bacillus and Lactobacillus
spp was detected within the feed additive group versus the antibiotic and control
groups. In conclusion, the feed additive group had a positive effect in the endogenous microbiota
of post-weaning pigs increasing both, the diversity of bacterial families and the abundance
of lactic acid bacteria during the post-weaning period.
This work was supported by a research contract of the University of Lleida with Nutriad International N.V. The funder provided support in the form of salaries for authors [Tim Goosens and Alvaro Bermejo], but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the "author contributions" section. |