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Title:	Comprehensive and quantitative analysis of G1 cyclins. A tool for studying the cell cycle
Author:	Ballega, Elisabet; Carballar Ruiz, María de los Reyes; Samper Martín, Bàrbara; Ricco Pacheco, Natalia; Ribeiro, Mariana P. C.; Bru Rullo, Samuel; Jiménez, Javier; Clotet Erra, Josep
Notes:	In eukaryotes, the cell cycle is driven by the actions of several cyclin dependent kinases (CDKs) and an array of regulatory proteins called cyclins, due to the cyclical expression patterns of the latter. In yeast, the accepted pattern of cyclin waves is based on qualitative studies performed by different laboratories using different strain backgrounds, different growing conditions and media, and different kinds of genetic manipulation. Additionally, only the subset of cyclins regulating Cdc28 was included, while the Pho85 cyclins were excluded. We describe a comprehensive, quantitative and accurate blueprint of G1 cyclins in the yeast Saccharomyces cerevisiae that, in addition to validating previous conclusions, yields new findings and establishes an accurate G1 cyclin blueprint. For the purposes of this research, we produced a collection of strains with all G1 cyclins identically tagged using the same and most respectful procedure possible. We report the contribution of each G1 cyclin for a broad array of growing and stress conditions, describe an unknown role for Pcl2 in heat-stress conditions and demonstrate the importance of maintaining the 3’UTR sequence of cyclins untouched during the tagging process.
Subject(s):	-Osmosi -Experiments científics -Expressió gènica -Cèl·lules -Ósmosis -Experimentos científicos -Expresión génica -Células -Ósmosis -Cells -Experiments -Gene expression -576 - Biologia cel·lular i sub-cel·lular. Citologia -61 - Medicina
Rights:	© 2019 Ballega et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. https://creativecommons.org/licenses/by/4.0/
Document type:	Article Article - Accepted version
Published by:	Public Library of Science
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