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Title:	Enhanced cell-material interactions through the biofunctionalization of polymeric surfaces with engineered peptides
Author:	Punet, Xavier; Mauchauffé, Rodolphe; Giannotti, Marina I.; Rodriguez Cabello, Jose Carlos; Sanz Carrasco, Fausto; Engel López, Elisabeth; Mateos Timoneda, Miguel Ángel; Planell Estany, Josep Anton
Other authors:	Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica; Institut de Bioenginyeria de Catalunya; Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits; Universidad de Valladolid
Abstract:	Research on surface modi fi cation of polymeric materials to guide the cellular activity in biomaterials designed for tissue engineering applications has mostly focused on the use of natural extracellular matrix (ECM) proteins and short peptides, such as RGD. However, the use of engineered proteins can gather the advantages of these strategies and avoid the main drawbacks. In this study, recombinant engineered proteins called elastin- like recombinamers (ELRs) have been used to functionalize poly(lactic) acid (PLA) model surfaces. The structure of the ELRs has been designed to include the integrin ligand RGDS and the cross-linking module VPGKG. Surface functionalization has been characterized and optimized by means of ELISA and atomic force microscopy (AFM). The results suggest that ELR functionalization creates a nonfouling canvas able to restrict unspeci fi c adsorption of proteins. Moreover, AFM analysis reveals the conformation and disposition of ELRs on the surface. Biological performance of PLA surfaces functionalized with ELRs has been studied and compared with the use of short peptides. Cell response has been assessed for di ff erent functionalization conditions in the presence and absence of the bovine serum albumin (BSA) protein, which could interfere with the surface − cell interaction by adsorbing on the interface. Studies have shown that ELRs are able to elicit higher rates of cell attachment, stronger cell anchorages and faster levels of proliferation than peptides. This work has demonstrated that the use of engineered proteins is a more e ffi cient strategy to guide the cellular activity than the use of short peptides, because they not only allow for better cell attachment and proliferation, but also can provide more complex properties such as the creation of nonfouling surfaces
Abstract:	Peer Reviewed
Subject(s):	-Àrees temàtiques de la UPC::Enginyeria dels materials -Biomechanics -Adsorption of proteins -Biofunctionalization -Biological performance -Bovine serum albumin proteins (BSA) -Cell-material interaction -Extracellular matrix protein -Surface Functionalization -Tissue engineering applications -Biomecànica -Enginyeria de teixits
Rights:	Attribution-NonCommercial-NoDerivs 3.0 Spain http://creativecommons.org/licenses/by-nc-nd/3.0/es/
Document type:	Article - Published version Article
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