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Title:	Biofilm oxygen profiling using an array of microelectrodes on a microfabricated needle
Author:	Moya Lara, Ana; Guimerà Villalba, Xavier; del Campo, F.J.; Prats-Alfonso, E.; Dorado Castaño, Antonio David; Baeza, Mireia; Villa, Rosa; Gabriel Buguña, David; Gamisans Noguera, Javier; Gabriel Buguña, Gemma
Other authors:	Universitat Politècnica de Catalunya. Departament d'Enginyeria Minera i Recursos Naturals; Universitat Politècnica de Catalunya. ERNMA - Enginyeria dels Recursos Naturals i Medi Ambient
Abstract:	Knowledge of bacterial activity inside biofilms is key to the optimization of applied biotechnologies. A novel dissolved oxygen microelectrode array (DO-MEA) sensor has been designed and fabricated on a needle using microelectromechanical systems technology, in order to provide a multipoint, simultaneous dissolved oxygen snapshot profile inside a biofilm. The sensor consisted of eleven gold disk electrodes with integrated counter (CE) and pseudo-reference (RE) electrodes. Three different sensor designs are fabricated, and their responses are fully characterized and evaluated under different environmental conditions. The developed sensor is validated against a commercial Clark-type microelectrode overcoming its drawbacks, by profiling a heterotrophic biofilm cultivated in a flat-plate bioreactor. The DO-MEA sensor will be improved with the protection of the electrodes and will be fabricated on a thinner substrate, for becoming a powerful tool for the research of many similar biological-based processes and applications.
Abstract:	Peer Reviewed
Abstract:	_
Subject(s):	-Àrees temàtiques de la UPC::Enginyeria química::Biotecnologia -Microelectronics -Biosensors -Dissolved Oxygen -Microelectrode Array -Microsensors -Bioprocess Monitoring -Profile -Biofilms -Microelectrònica -Biosensors
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Document type:	Conference Object
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