A novel process to create holographic nanostructures, through the production of silicone nanostructured printing plates, was recently developed. In order to optimize the process, was required an understanding of material and process interrelations. A characterization criteria was developed to analyze three nanostructured printing plates made respectively with the silicones, provided by Heraeus Kulzer, Correct Flow®, Light Flow® and Provil Novo®. The surface structure and the wettability of the silicones were analyzed. The surface structure could be only analyzed in the microscopic level, where no structural differences between the silicones were found. Nevertheless, their surfaces where affected by air bubbles and surface detachments in a different degree depending on the type of silicone. Light Flow® presented the largest number of air bubbles and surface detachments, with a respectively 9% and 14% of affected surface. Followed by Provil Novo® with a 4% and Correct Flow® with a 0,6% of damaged surface. Furthermore, was concluded that the air was introduced in the silicones at the time to deposit them above the printing rolls, therefore a change in the process methodology is required. Related with the wetting, Light Flow® proved to have the best wettability. A decrease of this property was noticed in the three silicone’s surfaces, due to an interaction with the liquid probes, as the measurements progressed. Thus, was concluded that a decrease in the nanostructured printings quality could be observed as result of the surface interaction with the ink.