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Title:	Influence of glycerol on poly(3-hydroxybutyrate) production by Cupriavidus necator and Burkholderia sacchari
Author:	Rodríguez-Contreras, Alejandra; Koller, Martin; Miranda-de Sousa Dias, Miguel; Calafell Monfort, Margarita; Braunegg, Gehart; Marqués Calvo, M. Soledad
Other authors:	Universitat Politècnica de Catalunya. Departament d'Òptica i Optometria; Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits; Technische Universität Graz. Institut für Biotechnology und Bioprozesstechnik
Abstract:	Glycerol is a co-product of many industrial processes and is generated in large quantities from different origins. In this study, glycerol is used as a cheap carbon source for the production of poly(3- hydroxybutyrate) (PHB) with two different collection strains, Cupriavidus necator and Burkholderia sacchari, in order to provide an alternative outlet for glycerol and produce value-added bioproducts. The objective of this work was to study the influence of this carbon source on their growth kinetics, on their polymer production, and on the molecular mass of the produced biopolymer. Therefore, fermentations in bioreactors were carried out with these strains. Different results for both strains were obtained showing, for the first time, a high cell dry mass and growth rate, when glycerol was used together with glucose in the fermentation with C. necator. In the first fermentation with B. sacchari using glycerol as a sole carbon source, the strain properly developed synthesizing PHB. The biopolymers obtained from both fermentations with glycerol showed low molecular masses about 300 kDa with a polydispersity of 4.72 with C. necator, and 200 kDa with polydispersity of 2.50 with B. sacchari.
Subject(s):	-Àrees temàtiques de la UPC::Enginyeria agroalimentària::Ciències de la terra i de la vida::Microbiologia -Àrees temàtiques de la UPC::Enginyeria química::Indústria dels processos químics::Reactors químics -Àrees temàtiques de la UPC::Física::Física molecular::Física de polímers -Poly-beta-hydroxybutyrate -Glycerin -Bioreactors -Fermentation -Microbial growth -Manufacturing processes -Biopolymers -Cupriavidus necator -Burkholderia sacchari -Poly(3-hydroxybutyrate) -Glycerol -Bioreactors -Molecular mass -Fed-Batch Culture -Fermentation -Microbial Growth -Glicerina -Bioreactors -Microbiologia -Biopolímers -Reactors químics
Rights:	http://creativecommons.org/licenses/by-nc-nd/3.0/es/
Document type:	Article - Submitted version Article
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