This report is Deliverable 2.3 Scenario Definition Document of the R-Wake project, resulting from WP2 activities. It provides the detailed methodology and definition of scenarios to be simulated and analysed in WP5 to generate the new knowledge and evidences of the level of risk and safety due to en-route wake vortex encounters (WVEs) hazards, and to support the proposal of enhancements of separation standards and methods to mitigate that hazards. The scenarios definition elaborates further the research approach identified in deliverable D2.1 System Specification, based in two steps: Step-1 focusses on micro scale modelling, in order to assess the WVE severity level and to obtain a Tolerability Risk Matrix (Safety case baseline); Step-2 focusses on macro-scale modelling of a number of ECAC-wide scenarios, in order to assess the risk and safety level in terms of probability (frequency) of events and mitigation, combining different patterns of traffic demand and ATM constraints, and different types of separation standards and mitigation methods. The R-Wake project addresses the SESAR 2020 Exploratory Research work-programme topic ER-07- 2015 - Separation Management and Separation Standards, within the area of Advanced Air Traffic Services.