Title:
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Rationally designed azobenzene photoswitches for efficient two-photon neuronal excitation
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Author:
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Cabré, Gisela; Garrido-Charle, Aida; Moreno, Miquel; Bosch, Miquel; Porta-de-la-Riva, Montserrat; Krieg, Michael; Gascón-Moya, Marta; Camarero, Núria; Gelabert, Ricard; Lluch, José M.; Busqué, Félix; Hernando, Jordi; Gorostiza, Pau; Alibés, Ramon
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Abstract:
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Manipulation of neuronal activity using two-photon excitation of azobenzene photoswitches
with near-infrared light has been recently demonstrated, but their practical use in neuronal
tissue to photostimulate individual neurons with three-dimensional precision has been
hampered by firstly, the low efficacy and reliability of NIR-induced azobenzene photoisomerization compared to one-photon excitation, and secondly, the short cis state lifetime of
the two-photon responsive azo switches. Here we report the rational design based on theoretical calculations and the synthesis of azobenzene photoswitches endowed with both high
two-photon absorption cross section and slow thermal back-isomerization. These compounds provide optimized and sustained two-photon neuronal stimulation both in lightscattering brain tissue and in Caenorhabditis elegans nematodes, displaying photoresponse
intensities that are comparable to those achieved under one-photon excitation. This finding
opens the way to use both genetically targeted and pharmacologically selective azobenzene
photoswitches to dissect intact neuronal circuits in three dimensions. |
Abstract:
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Peer Reviewed |
Subject(s):
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-Àrees temàtiques de la UPC::Física -Neurons -neuronal excitation -Neurones |
Rights:
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Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
Document type:
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Article - Published version Article |
Published by:
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Nature
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