dc.contributor.author |
Tamarit Sumalla, Jordi |
dc.contributor.author |
Mulliez, Etienne |
dc.contributor.author |
Meier, Christian |
dc.contributor.author |
Trautwein, Alfred |
dc.contributor.author |
Fontecave, Marc |
dc.date |
2016-03-30T08:17:26Z |
dc.date |
2016-03-30T08:17:26Z |
dc.date |
1999 |
dc.identifier |
0021-9258 |
dc.identifier |
http://hdl.handle.net/10459.1/56767 |
dc.identifier |
https://doi.org/10.1074/jbc.274.44.31291 |
dc.identifier.uri |
http://hdl.handle.net/10459.1/56767 |
dc.description |
For deoxyribonucleotide synthesis during anaerobic
growth, Escherichia coli cells depend on an oxygen-sensitive
class III ribonucleotide reductase. The enzyme
system consists of two proteins: protein a, on which
ribonucleotides bind and are reduced, and protein b, of
which the function is to introduce a catalytically essential
glycyl radical on protein a. Protein b can assemble
one [4Fe-4S] center per polypeptide enjoying both the
[4Fe-4S]21 and [4Fe-4S]11 redox state, as shown by iron
and sulfide analysis, Mo¨ssbauer spectroscopy (d 5 0.43
mmzs21
, DEQ 5 1.0 mmzs21
, [4Fe-4S]21), and EPR spectroscopy
(g 5 2.03 and 1.93, [4Fe-4S]11). This iron center
is sensitive to oxygen and can decompose into stable
[2Fe-2S]21 centers during exposure to air. This degraded
form is nevertheless active, albeit to a lesser extent because
of the conversion of the cluster into [4Fe-4S] forms
during the strongly reductive conditions of the assay.
Furthermore, protein b has the potential to activate
several molecules of protein a, suggesting that protein b
is an activating enzyme rather than a component of an
a2b2 complex as previously claimed. |
dc.language |
eng |
dc.publisher |
American Society for Biochemistry and Molecular Biology |
dc.relation |
Reproducció del document publicat a https://doi.org/10.1074/jbc.274.44.31291 |
dc.relation |
Journal of Biological Chemistry, 1999, Vol. 274, núm. 44, p. 31291-31296 |
dc.rights |
(c) The American Society for Biochemistry and Molecular Biology, 1999 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.title |
The Anaerobic Ribonucleotide Reductase from Escherichia coli |
dc.type |
article |
dc.type |
publishedVersion |