Protein carbonylation: proteomics, specificity and relevance to aging

Abstract

Detection and quantification of protein carbonyls present in biological samples has become a popular, albeit indirect, method to determine the existence of oxidative stress. Moreover, the rise of proteomics has allowed the identification of the specific proteins targeted by protein carbonylation. This review discusses these methodologies and proteomic strategies and then focuses on the relationship between protein carbonylation and aging and the parameters that may explain the increased sensitivity of certain proteins to protein carbonylation.

This work has been supported by grants BFU 2010-19193, BFU2010-17387 and CSD2007-20 Consolider Ingenio 2010 from the Ministerio de Ciencia e Innovación (Spain) and SGR2009-00196 from the Generalitat de Catalunya.