Differences in free amino acid profile of non-thermally treated tomato and strawberry juices

Abstract

The amino acids profiles of tomato and strawberry juices subjected to high intensity pulsed electric fields (HIPEF) treatment were evaluated and compared to those of thermally treated juices. Both HIPEF and thermal treatments (TT) depleted the initial content of total free amino acids of tomato juice. However, HIPEF-treated juices exhibited higher concentration (769 mg/L) than thermally treated juices (750 mg/L). HIPEF treatment enhanced the concentration of phenylalanine (27%), glutamic acid (6.8%), valine (6.3%), serine (5.5%) and alanine (4.8%), and in turn, the total free amino content of strawberry juice. In contrast, TT caused a reduction in the content of most amino acids compared to fresh juice. Although total free amino acids in treated tomato juice were substantially enhanced during storage, the content of some minor amino acids (proline, leucine, valine, isoleucine, arginine, lysine, phenylalanine and methionine) decreased over time. Regarding strawberry, HIPEF-treated juices exhibited higher amino acid content than thermally treated juices during storage. Individual and total amino acid concentrations increased during the storage, except for leucine concentrations, which significantly decreased (23%) with time. Hence, HIPEF could be considered a potential preservation and processing method to obtain shelf-stable fruit juices with high nutritional value.

This study has been carried out with financial support from the Commission of the European Communities, Framework 6, Priority 5 “Food Quality and Safety”, Integrated Project NovelQ FP6-CT-2006-015710. This work was also supported by the Interministerial Commission for Science and Technology (CICYT) of the Ministerio de Educación y Ciencia (Spain) through the Project ALI 2005-05768. Isabel Odriozola-Serrano thanks the Agència de Gestió d’Ajuts Universitaris i de Recerca of the Generalitat de Catalunya (Spain) and the European Social Fund for the predoctoral grant. Prof. Olga Martín-Belloso thanks to the Institució Catalana de Recerca i Estudis Avançats (ICREA) for the Academia Award 2008.