dc.contributor.author |
Rivera Vélez, Sol Maiam |
dc.contributor.author |
Vilaró, Francisca |
dc.contributor.author |
Zhu, Changfu |
dc.contributor.author |
Bai, Chao |
dc.contributor.author |
Farré Martinez, Gemma |
dc.contributor.author |
Christou, Paul |
dc.contributor.author |
Canela i Garayoa, Ramon |
dc.date |
2016-12-20T10:56:59Z |
dc.date |
2013 |
dc.date |
10000-01-01 |
dc.identifier |
0021-8561 |
dc.identifier |
http://hdl.handle.net/10459.1/58873 |
dc.identifier |
https://doi.org/10.1021/jf400694z |
dc.identifier.uri |
http://hdl.handle.net/10459.1/58873 |
dc.description |
A fast method was developed to determine carotenoid content in transgenic maize seeds. The analysis was carried out using an ultrahigh-pressure liquid chromatograph coupled to a photodiode array detector and a mass spectrometer (UHPLC-PDA-MS/MS). Sixteen carotenoid pigments were detected and quantified in <13 min. In addition, it was possible to obtain good resolution of both polar xanthophylls and nonpolar carotenes. The method exhibited (a) a high degree of repeatability (%RSD < 13%), (b) linear calibration curves (R2 > 0.9952), (c) satisfactory recoveries for most of the pigments (between 82 and 108%), and (d) low detection (from 0.02 to 0.07 μg/mL) and quantification limits (from 0.05 to 0.20 μg/mL) (LOD and LOQ, respectively). The methodology was applied to the analysis of transgenic maize lines TM1, TM2, and TM3, expressing several carotenogenic genes. |
dc.description |
We thank the Comissionat per a Universitats i Recerca del
DIUE de la Generalitat de Catalunya (Barcelona, Spain) and
the European Social Fund (ESF) for a Ph.D. fellowship to
S.M.R. This work was supported by the University of Lleida,
Ministry of Science and Innovation (MICINN), Spain
(BFU200761413 and CTQ2009-14699-C02-01), and an ERC
Advanced Grant (BIOFORCE) to P.C. |
dc.language |
eng |
dc.publisher |
American Chemical Society |
dc.relation |
MIECI/PN2004-2007/BFU2007-61413 |
dc.relation |
MICINN/PN2008-2011/CTQ2009-14699-C02-01 |
dc.relation |
Reproducció del document publicat a https://doi.org/10.1021/jf400694z |
dc.relation |
Journal of Agricultural and Food Chemistry, 2013, vol. 61, núm. 22, p. 5279-5285 |
dc.relation |
info:eu-repo/grantAgreement/EC/FP7/232933 |
dc.rights |
(c) American Chemical Society, 2013 |
dc.rights |
info:eu-repo/semantics/restrictedAccess |
dc.subject |
Carotenoids |
dc.subject |
Maize |
dc.subject |
Ultrahigh-pressure liquid chromatography (UHPLC) |
dc.title |
Fast quantitative method for the analysis of carotenoids in transgenic maize |
dc.type |
article |
dc.type |
publishedVersion |