Universitat de Lleida
The compound 2,5-di(hydroxymethyl)furan (DHMF) is a high-value chemical block that can be synthesized from 5-hydroxymethylfurfural (HMF), a platform chemical that results from the dehydration of biomass-derived carbohydrates. In this work, the HMF biotransformation capability of different Fusarium species was evaluated, and F. striatum was selected to produce DHMF. The effects of the inoculum size, glucose concentration and pH of the media over DHMF production were evaluated by a 23 factorial design. A substrate feeding approach was found suitable to overcome the toxicity effect of HMF towards the cells when added at high concentrations (>75 mM). The process was successfully scaled-up at bioreactor scale (1.3 L working volume) with excellent DHMF production yields (95%) and selectivity (98%). DHMF was purified from the reaction media with high recovery and purity by organic solvent extraction with ethyl acetate.
This work was partially supported by the Spanish government (PID2019-110735RB-C21, MICIN/FEDER) and the Catalan Government (FI_B1_00135).
English
Biocatalysis; Whole cells; Biotransformation; Platform chemical; Biomass; Bioreactor
MDPI
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110735RB-C21/ES/APLICACION DE DISOLVENTES IONICOS EUTECTICOS BASADOS EN PRODUCTOS NATURALES A LA VALORIZACION DE BIOMASA NO ALIMENTARIA/
Reproducció del document publicat a: https://doi.org/10.3390/catal11020216
Catalysts, 2021, vol. 11, núm. 2, p. 216
cc-by (c) Millán, Alberto et al., 2021
http://creativecommons.org/licenses/by/4.0/
Documents de recerca [17848]
