Sistema de Salut de Catalunya
[Villar-Hernández R] Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Barcelona, Spain. CIBER Enfermedades Respiratorias, CIBERES, Instituto de Salud Carlos III, Madrid, Spain. Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain. R&D Department, Genome Identification Diagnostics (GenID) GmbH, Strassberg, Germany. [Latorre I] Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Barcelona, Spain. CIBER Enfermedades Respiratorias, CIBERES, Instituto de Salud Carlos III, Madrid, Spain. Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain. [Noguera-Julian A] Malalties Infeccioses i Resposta Inflamatòria Sistèmica en Pediatria, Servei de Malalties Infeccioses i Patologia Importada, Institut de Recerca Sant Joan de Déu, Barcelona, Spain. Red de Investigación Translacional en Infectología Pediátrica, RITIP, Madrid, Spain. Departament de Cirurgia i Especialitats Medicoquirúrgiques, Universitat de Barcelona, Barcelona, Spain. CIBER de Epidemiología y Salud Pública, CIBERESP, Instituto de Salud Carlos III, Madrid, Spain. [Martínez-Planas A] Malalties Infeccioses i Resposta Inflamatòria Sistèmica en Pediatria, Servei de Malalties Infeccioses i Patologia Importada, Institut de Recerca Sant Joan de Déu, Barcelona, Spain. Red de Investigación Translacional en Infectología Pediátrica, RITIP, Madrid, Spain. [Minguell L, Vallmanya T] Department of Pediatrics, Hospital Universitari Arnau de Vilanova, Lleida, Spain. [Galea Y] Servei de Pneumologia, Hospital General de Granollers, Granollers, Spain
Hospital General de Granollers
2024-01-26T14:04:40Z
2024-01-26T14:04:40Z
2023-12-18
Nontuberculous mycobacteria; Tuberculosis; Glycopeptidolipids
Micobacterias no tuberculosas; Tuberculosis; Glicopeptidolípidos
Micobacteris no tuberculosos; Tuberculosi; Glicopeptidolípids
Background: Diagnosis of nontuberculous mycobacteria (NTM) infections remains a challenge. In this study, we describe the evaluation of an immunological NTM-interferon (IFN)-γ release assay (IGRA) that we developed using glycopeptidolipids (GPLs) as NTM-specific antigens. Methods: We tested the NTM-IGRA in 99 samples from pediatric patients. Seventy-five were patients with lymphadenitis: 25 were NTM confirmed, 45 were of unknown etiology but compatible with mycobacterial infection and 5 had lymphadenitis caused by an etiologic agent other than NTM. The remaining 24 samples were from control individuals without lymphadenitis (latently infected with M. tuberculosis, uninfected controls and active tuberculosis patients). Peripheral blood mononuclear cells were stimulated overnight with GPLs. Detection of IFN-γ producing cells was evaluated by enzyme-linked immunospot assay. Results: NTM culture-confirmed lymphadenitis patient samples had a significantly higher response to GPLs than the patients with lymphadenitis of unknown etiology but compatible with mycobacterial infection (P < 0.001) and lymphadenitis not caused by NTM (P < 0.01). We analyzed the response against GPLs in samples from unknown etiology lymphadenitis but compatible with mycobacterial infection cases according to the tuberculin skin test (TST) response, and although not statistically significant, those with a TST ≥5 mm had a higher response to GPLs when compared with the TST <5 mm group. Conclusions: Stimulation with GPLs yielded promising results in detecting NTM infection in pediatric patients with lymphadenitis. Our results indicate that the test could be useful to guide the diagnosis of pediatric lymphadenitis. This new NTM-IGRA could improve the clinical handling of NTM-infected patients and avoid unnecessary misdiagnosis and treatments.
Article
Accepted version
English
Micobacteriosis; Tuberculosi; Antibiòtics; ORGANISMS::Bacteria::Gram-Positive Bacteria::Actinobacteria::Actinomycetales::Mycobacteriaceae::Mycobacterium::Nontuberculous Mycobacteria; CHEMICALS AND DRUGS::Carbohydrates::Glycoconjugates::Glycopeptides; DISEASES::Hemic and Lymphatic Diseases::Lymphatic Diseases::Lymphadenitis; ORGANISMOS::Bacteria::bacterias grampositivas::Actinobacteria::Actinomycetales::Mycobacteriaceae::Mycobacterium::micobacterias no tuberculosas; COMPUESTOS QUÍMICOS Y DROGAS::hidratos de carbono::glicoconjugados::glicopéptidos; ENFERMEDADES::enfermedades hematológicas y linfáticas::enfermedades linfáticas::linfadenitis
Lippincott, Williams & Wilkins
The Pediatric Infectious Disease Journal;
https://doi.org/10.1097/INF.0000000000004211
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
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