dc.contributor
Institut Català de la Salut
dc.contributor
[L’Imperio V, Cazzaniga G, Mannino M, Bono F, Seminati D] Pathology, IRCCS Fondazione San Gerardo dei Tintori and Centro di Medicina Digitale, Dipartimento di Medicina e Chirurgia, University of Milano-Bicocca, Italy. [Capitoli G] Bicocca Bioinformatics Biostatistics and Bioimaging B4 Center, School of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy. [Temprana-Salvador J] Servei d’Anatomia Patològica, Vall d’Hebron Hospital Universitari, Barcelona, Spain
dc.contributor
Vall d'Hebron Barcelona Hospital Campus
dc.contributor.author
L'Imperio, Vincenzo
dc.contributor.author
CAPITOLI, GIULIA
dc.contributor.author
Cazzaniga, Giorgio
dc.contributor.author
Mannino, Mauro
dc.contributor.author
Bono, Francesca
dc.contributor.author
Seminati, Davide
dc.contributor.author
Temprana Salvador, Jordi
dc.date.accessioned
2025-10-24T10:41:53Z
dc.date.available
2025-10-24T10:41:53Z
dc.date.issued
2025-10-22T12:27:57Z
dc.date.issued
2025-10-22T12:27:57Z
dc.identifier
L’Imperio V, Capitoli G, Cazzaniga G, Mannino M, Bono F, Seminati D, et al. The routine use of a digital tool for the tumor cell fraction quantification in molecular pathology: an international validation of QuANTUM. Pathologica. 2025 Jun;117(3):269–77.
dc.identifier
http://hdl.handle.net/11351/13917
dc.identifier
10.32074/1591-951X-1100
dc.identifier.uri
https://hdl.handle.net/11351/13917
dc.description.abstract
Molecular pathology; Non-small cell lung cancer; Tumor cell fraction
dc.description.abstract
Patología molecular; Cáncer de pulmón de células no pequeñas; Fracción de células tumorales
dc.description.abstract
Patologia molecular; Càncer de pulmó de cèl·lules no petites; Fracció de cèl·lules tumorals
dc.description.abstract
Objective: The absolute and relative quantification of tumor cell fraction (TCF) in tissue samples for molecular pathology testing is time-consuming and poorly reproducible.
Methods: Here we report the results of an international survey on non-small cell lung cancer (NSCLC), validating the Qupath Analysis of Nuclei from Tumor to Uniform Molecular tests (QuANTUM) automated computational pipeline for TCF quantification.
Results: The TCF obtained with QuANTUM is reliable, as demonstrated by the comparison with the manual counting of cells (ground truth, GT) in cell blocks, small biopsies and surgical specimens (overall correlation of 0.89). The visual evaluation of QuANTUM-processed images increased the pathologists' agreement with GT and QuANTUM of +0.16, +0.21, +0.09 and +0.17, +0.29, +0.21 across the three sample types, respectively. An overall increase in cases classified as containing ≥100 tumor cells for all sample types was noted after QuANTUM (from 75 cases, 63% to 96 cases, 80% among cell blocks, p = 0.003).
Conclusions: QuANTUM is an easy-to-use and reliable tool for the TCF assessment and its employment significantly modifies the visual estimation by pathologists, improving the assessment of NSCLC cases for molecular analysis.
dc.description.abstract
The work has been funded by the project in the Italian Ministry of the University MUR Dipartimenti di Eccellenza 2023-2027 (l. 232/2016, art. 1, commi 314-337). This work was partially funded by the National Plan for NRRP Complementary Investments (PNC, established with the decree-law 6 May 2021, n. 59, converted by law n. 101 of 2021) in the call for the funding of research initiatives for technologies and innovative trajectories in the health and care sectors (Directorial Decree n. 931 of 06-06-2022) - project n. PNC0000003 - AdvaNced Technologies for Human-centrEd Medicine (project acronym: ANTHEM).
dc.format
application/pdf
dc.publisher
Pacini Editore
dc.relation
Pathologica;117(3)
dc.relation
https://doi.org/10.32074/1591-951X-1100
dc.rights
Attribution-NonCommercial-NoDerivatives 4.0 International
dc.rights
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights
info:eu-repo/semantics/openAccess
dc.subject
Pulmons - Càncer - Diagnòstic
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Pulmons - Càncer - Aspectes moleculars
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Citologia - Tècnica
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DISEASES::Neoplasms::Neoplasms by Site::Thoracic Neoplasms::Respiratory Tract Neoplasms::Lung Neoplasms::Bronchial Neoplasms::Carcinoma, Bronchogenic::Carcinoma, Non-Small-Cell Lung
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Other subheadings::Other subheadings::/diagnosis
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ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Cytological Techniques::Cell Count
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DISCIPLINES AND OCCUPATIONS::Natural Science Disciplines::Biological Science Disciplines::Biochemistry::Molecular Biology::Molecular Medicine::Pathology, Molecular
dc.subject
ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias torácicas::neoplasias del tracto respiratorio::neoplasias pulmonares::neoplasias de los bronquios::carcinoma broncogénico::carcinoma de pulmón de células no pequeñas
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Otros calificadores::Otros calificadores::/diagnóstico
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TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::diagnóstico::técnicas y procedimientos diagnósticos::técnicas de laboratorio clínico::técnicas citológicas::recuento de células
dc.subject
DISCIPLINAS Y OCUPACIONES::disciplinas de las ciencias naturales::disciplinas de las ciencias biológicas::bioquímica::biología molecular::medicina molecular::patología molecular
dc.title
The routine use of a digital tool for the tumor cell fraction quantification in molecular pathology: an international validation of QuANTUM
dc.type
info:eu-repo/semantics/article
dc.type
info:eu-repo/semantics/publishedVersion
