Sistema de Salut de Catalunya
Institut Català de la Salut
[Villar-Hernández R, Latorre I] Servei de Microbiología, Hospital Universitari Germans Trias i Pujol, Badalona, Spain. Institut d'Investigació Germans Trias i Pujol, Badalona, Spain. CIBER Enfermedades Respiratorias, Instituto de Salud Carlos III, Badalona, Spain. Universitat Autònoma de Barcelona, Badalona, Spain. [De Souza-Galvão ML, Jiménez MA] Unitat de Tuberculosi Vall d'Hebron-Drassanes, Hospital Universitari Vall d'Hebron, Barcelona, Spain. [Ruiz-Manzano J] CIBER Enfermedades Respiratorias, Instituto de Salud Carlos III, Badalona, Spain. Universitat Autònoma de Barcelona, Badalona, Spain. Servei de Pneumologia, Hospital Universitari Germans Trias i Pujol, Badalona, Spain. [Pilarte J, Cantos A] Unitat de Tuberculosi Vall d'Hebron-Drassanes, Hospital Universitari Vall d'Hebron, Barcelona, Spain. [Altet N] Unitat de Tuberculosi Vall d'Hebron-Drassanes, Hospital Universitari Vall d'Hebron, Barcelona, Spain. Unidad Clínica de Tratamiento Directamente Observado "Serveis Clinics”, Barcelona, Spain. CIBER de Epidemiología y Salud Pública, Instituto de Salud Carlos III, Badalona, Spain.
Vall d'Hebron Barcelona Hospital Campus
2019-04-11T08:49:49Z
2019-04-11T08:49:49Z
2019-03-08
IP-10; Tuberculosis; Mail
IP-10; Tuberculosi; Correu
IP-10; Tuberculosis; Correo
The aim of this study was to test the use of IP-10 detection in dried plasma from contact studies individuals (contacts of smear positive patients), by comparing it with IP-10 and IFN-γ detection in direct plasma, to establish IP-10 detection in DPS as a useful assay for LTBI diagnosis. Whole blood samples were collected from 80 subjects: 12 with active tuberculosis (TB), and 68 from contact studies. The amount of IFN-γ produced by sensitized T cells was determined in direct plasma by QuantiFERON Gold In-Tube test. IP-10 levels were determined in direct and dried plasma by an in-house ELISA. For dried plasma IP-10 determination, two 25 µl plasma drops were dried in Whatman903 filter paper and sent by mail to the laboratory. Regarding TB patients, 100.0%, 91.7% and 75.0% were positive for IFN-γ detection and IP-10 detection in direct and dried plasma, respectively. In contacts, 69.1%, 60.3% and 48.5% had positive results after IFN-γ and IP-10 in direct and dried plasma, respectively. The agreement among in vitro tests was substantial and IP-10 levels in direct and dried plasma were strongly correlated (r = 0.897). In conclusion, IP-10 detection in dried plasma is a simple and safe method that would help improve LTBI management.
Article
Published version
English
Tuberculosi - Diagnòstic; Quimiocines; DISEASES::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Tuberculosis::Latent Tuberculosis; Other subheadings::Other subheadings::/diagnosis; CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines::Chemokines::Chemokines, CXC::Chemokine CXCL10; ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES AND EQUIPMENT::Investigative Techniques::Epidemiologic Methods::Contact Tracing; ENFERMEDADES::infecciones bacterianas y micosis::infecciones bacterianas::infecciones por bacterias grampositivas::infecciones por Actinomycetales::micobacteriosis::tuberculosis::tuberculosis latente; Otros calificadores::Otros calificadores::/diagnóstico; COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::péptidos::péptidos y proteínas de señalización intercelular::citocinas::quimiocinas::quimiocinas CXC::quimiocina CXCL10; TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::métodos epidemiológicos::rastreo de contactos
Nature Research
Scientific Reports;9(1)
https://www.nature.com/articles/s41598-019-40778-1
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
Articles científics - HVH [3440]
