Title:
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CPT1C promotes human mesenchymal stem cells survival under glucose deprivation through the modulation of autophagy
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Author:
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Roa Mansergas, Xavier; Fadó Andrés, Rut; Atari Abouassi, Maher; Mir, Joan Francesc; Muley Vilamú, Helena; Serra, Dolors; Casals i Farré, Núria
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Notes:
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Human mesenchymal stem cells (hMSCs) are widely used in regenerative medicine. In some
applications, they must survive under low nutrient conditions engendered by avascularity. Strategies to
improve hMSCs survival may be of high relevance in tissue engineering. Carnitine palmitoyltransferase
1 C (CPT1C) is a pseudoenzyme exclusively expressed in neurons and cancer cells. In the present
study, we show that CPT1C is also expressed in hMSCs and protects them against glucose starvation,
glycolysis inhibition, and oxygen/glucose deprivation. CPT1C overexpression in hMSCs did not increase
fatty acid oxidation capacity, indicating that the role of CPT1C in these cells is different from that
described in tumor cells. The increased survival of CPT1C-overexpressing hMSCs observed during
glucose deficiency was found to be the result of autophagy enhancement, leading to a greater number
of lipid droplets and increased intracellular ATP levels. In fact, inhibition of autophagy or lipolysis was
observed to completely block the protective effects of CPT1C. Our results indicate that CPT1C-mediated
autophagy enhancement in glucose deprivation conditions allows a greater availability of lipids to be
used as fuel substrate for ATP generation, revealing a new role of CPT1C in stem cell adaptation to low
nutrient environments. |
Subject(s):
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-Cèl·lules mare -Stem cells -Células madre -Medicina regenerativa -Regenerative medicine -CPT1C -61 |
Rights:
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http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
Document type:
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Article Article - Accepted version |
Published by:
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Nature Research
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