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Title:	MyoD induces ARTD1 and nucleoplasmic poly-ADP-ribosylation during fibroblast to myoblast transdifferentiation
Author:	Bisceglie, Lavinia; Hopp, Ann-Katrin; Gunasekera, Kapila; Wright, Roni H.G.; Le Dily, François; Vidal, Enrique; Dall'Agnese, Alessandra; Caputo, Luca; Nicoletti, Chiara; Puri, Pier Lorenzo; Beato, Miguel; Hottiger, Michael O.
Notes:	While protein ADP-ribosylation was reported to regulate differentiation and dedifferentiation, it has so far not been studied during transdifferentiation. Here, we found that MyoD-induced transdifferentiation of fibroblasts to myoblasts promotes the expression of the ADP-ribosyltransferase ARTD1. Comprehensive analysis of the genome architecture by Hi-C and RNA-seq analysis during transdifferentiation indicated that ARTD1 locally contributed to A/B compartmentalization and coregulated a subset of MyoD target genes that were however not sufficient to alter transdifferentiation. Surprisingly, the expression of ARTD1 was accompanied by the continuous synthesis of nuclear ADP ribosylation that was neither dependent on the cell cycle nor induced by DNA damage. Conversely to the H2O2-induced ADP-ribosylation, the MyoD-dependent ADP-ribosylation was not associated to chromatin but rather localized to the nucleoplasm. Together, these data describe a MyoD-induced nucleoplasmic ADP-ribosylation that is observed particularly during transdifferentiation and thus potentially expands the plethora of cellular processes associated with ADP-ribosylation.
Subject(s):	-Ciències biològiques -Biologia cel·lular -Biologia del desenvolupament -Biologia molecular -Ciencias Biologicas -Biología del desarrollo -Biología molecular -Biología celular -Biological sciences -Cell biology -Developmental biology -Molecular biology -57
Rights:	© 2021 The Author(s).This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). https://creativecommons.org/licenses/by-nc-nd/4.0/
Document type:	Article Article - Published version
Published by:	Elsevier
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