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Title:
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Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates
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Author:
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Ciapetti, Gabriela; Pompo, Gemma di; Avnet, Sofia; Martini, Desirée; Díez Escudero, Anna; Montufar Jiménez, Edgar Benjamin; Ginebra Molins, Maria Pau; Baldini, Nicola
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Other authors:
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Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica; Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits; Istituto Ortopedico Rizzoli |
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Abstract:
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The design of synthetic bone grafts to foster bone formation is a challenge in regenerative medicine. Understanding the interaction of bone substitutes with osteoclasts is essential, since osteoclasts not only drive a timely resorption of the biomaterial, but also trigger osteoblast activity.
In this study, the adhesion and differentiation of human blood-derived osteoclast precursors (OCP) on two different micro-nanostructured biomimetic hydroxyapatite materials consisting in coarse (HA-C) and fine HA (HA-F) crystals, in comparison with sintered stoichiometric HA (sin-HA, reference material), were investigated. Osteoclasts were induced to differentiate by RANKL-containing supernatant using cell/substrate direct and indirect contact systems, and calcium (Ca++) and phosphorus (P5+) in culture medium were measured.
We observed that OCP adhered to the experimental surfaces, and that osteoclast-like cells formed at a rate influenced by the micro- and nano-structure of HA, which also modulate extracellular Ca++.
Qualitative differences were found between OCP on biomimetic HA-C and HA-F and their counterparts on plastic and sin-HA. On HA-C and HA-F cells shared typical features of mature osteoclasts, i.e. podosomes, multinuclearity, tartrate acid phosphatase (TRAP)-positive staining, and TRAP5b-enzyme release. However, cells were less in number compared to those on plastic or on sin-HA, and they did not express some specific osteoclast markers. In conclusion, blood-derived OCP are able to attach to biomimetic and sintered HA substrates, but their subsequent fusion and resorptive activity are hampered by surface micro-nano-structure. Indirect cultures suggest that fusion of OCP is sensitive to topography and to extracellular calcium. |
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Subject(s):
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-Àrees temàtiques de la UPC::Enginyeria dels materials
-Bone resorption
-Hydroxyapatite
-Osteoclasts
-Topography
-Osteoclasts
-Hydroxyapatite
-Differentiation
-Topography
-Ionic exchange
-Bone resorption
-Hidroxiapatita
-Osteopatia |
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Rights:
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Document type:
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Article - Draft
Article |
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