Universitat Autònoma de Barcelona
An impedimetric label-free genosensor for high sensitive DNA detection is developed. This system is based on a screen-printed carbon electrode modified with the thionine layer and iridium oxide nanoparticles (IrO₂ NP/). An aminated oligonucleotide probe is immobilized on the IrO₂ NP/polythionine modified electrode and ethanolamine was used as a blocking agent. Different diluted PCR amplified DNA samples have been detected. The selectivity and reproducibility of this system are studied and the system was highly reproducible with RSD ≈ 15% and sensitive enough while using 2% of ethanolamine during the blocking step employed for genosensor preparation.
English
Blocking agents; DNA detection; Genosensors; Iridium oxides; Modified electrodes; Oligonucleotide probes; Reproducibilities; Screen-printed carbon electrodes
European Commission 315653
Ministerio de Economía y Competitividad SEV-2013-0295
Ministerio de Economía y Competitividad MAT2011-25870
Journal of materials chemistry B ; Vol. 3, issue 26 (July 2015), p. 5166-5171
open access
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