Título:
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Genome-wide analysis of factors affecting transcription elongation and DNA repair : a new role for PAF and Ccr4-not in transcription-coupled repair
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Autor/a:
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Gaillard, Hélène; Tous, Cristina; Botet, Javier; González Aguilera, Cristina; Quintero, María José; Viladevall Masbernat, Laia; García Rubio, María L.; Rodríguez Gil, Alfonso; Marín Rodríguez, Antonio; Ariño Carmona, Joaquín; Revuelta, José Luis; Chávez, Sebastián; Aguilera, Andrés
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Abstract:
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RNA polymerases frequently deal with a number of obstacles during transcription elongation that need to be removed for transcription resumption. One important type of hindrance consists of DNA lesions, which are removed by transcription-coupled repair (TC-NER), a specific sub-pathway of nucleotide excision repair. To improve our knowledge of transcription elongation and its coupling to TC-NER, we used the yeast library of non-essential knock-out mutations to screen for genes conferring resistance to the transcription-elongation inhibitor mycophenolic acid and the DNA-damaging agent 4-nitroquinoline-N-oxide. Our data provide evidence that subunits of the SAGA and Ccr4-Not complexes, Mediator, Bre1, Bur2, and Fun12 affect transcription elongation to different extents. Given the dependency of TC-NER on RNA Polymerase II transcription and the fact that the few proteins known to be involved in TC-NER are related to transcription, we performed an in-depth TC-NER analysis of a selection of mutants. We found that mutants of the PAF and Ccr4-Not complexes are impaired in TC-NER. This study provides evidence that PAF and Ccr4-Not are required for efficient TC-NER in yeast, unraveling a novel function for these transcription complexes and opening new perspectives for the understanding of TC-NER and its functional interconnection with transcription elongation. |
Materia(s):
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-Transcription factors -DNA damage -Damage mechanics -Yeast -Mutation -Ultraviolet radiation -DNA repair |
Derechos:
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open access
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Tipo de documento:
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Article |
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Uri:
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https://ddd.uab.cat/record/112594
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