Título:
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Irreversible electroporation of the liver : is there a safe limit to the ablation volume?
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Autor/a:
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Sánchez-Velázquez, Patricia; Castellví, Quim; Villanueva Garatachea, Alberto; Quesada, Rita; Pañella, Clara; Cáceres, Marta; Dorcaratto, Dimitri; Andaluz Martínez, Anna; Moll Sánchez, Xavier; Trujillo Guillén, Macarena; Berjano, Enrique; Grande, L; Ivorra Cano, Antoni; Burdío, Fernando
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Abstract:
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Irreversible electroporation is a fast-growing liver ablation technique. Although safety has been well documented in small ablations, our aim is to assess its safety and feasibility when a large portion of liver is ablated. Eighty-seven mice were subjected to high voltage pulses directly delivered across parallel plate electrodes comprising around 40% of mouse liver. One group consisted in 55 athymicnude, in which a tumor from the KM12C cell line was grown and the other thirty-two C57-Bl6 nontumoral mice. Both groups were subsequently divided into subsets according to the delivered field strength (1000V/cm, 2000V/cm) and whether or not they received anti-hyperkalemia therapy. Early mortality (less than 24hours post-IRE) in the 2000V/cm group was observed and revealed considerably higher mean potassium levels. In contrast, the animals subjected to a 2000V/cm field treated with the anti-hyperkalemia therapy had higher survival rates (OR=0.1, 95%CI=0.02-0.32, p<0.001). Early mortality also depended on the electric field magnitude of the IRE protocol, as mice given 1000V/cm survived longer than those given 2000V/cm (OR=4.7, 95%CI=1.8-11.8, p=0.001). Our findings suggest that ionic disturbances, mainly due to potassium alterations, should be warned and envisioned when large volume ablations are performed by IRE. |
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open access
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Article |
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Uri:
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https://ddd.uab.cat/record/205998
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