Título:
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Structural analysis of SARS-CoV-2 genome and predictions of the human interactome
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Autor/a:
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Vandelli, Andrea; Monti, Michele; Milanetti, Edoardo; Armaos, Alexandros; Rupert, Jakob; Zacco, Elsa; Bechara, Elias; Delli Ponti, Riccardo; Tartaglia, Gian Gaetano
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Abstract:
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Specific elements of viral genomes regulate interactions within host cells. Here, we calculated the secondary structure content of >2000 coronaviruses and computed >100 000 human protein interactions with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The genomic regions display different degrees of conservation. SARS-CoV-2 domain encompassing nucleotides 22 500-23 000 is conserved both at the sequence and structural level. The regions upstream and downstream, however, vary significantly. This part of the viral sequence codes for the Spike S protein that interacts with the human receptor angiotensin-converting enzyme 2 (ACE2). Thus, variability of Spike S is connected to different levels of viral entry in human cells within the population. Our predictions indicate that the 5' end of SARS-CoV-2 is highly structured and interacts with several human proteins. The binding proteins are involved in viral RNA processing, include double-stranded RNA specific editases and ATP-dependent RNA-helicases and have strong propensity to form stress granules and phase-separated assemblies. We propose that these proteins, also implicated in viral infections such as HIV, are selectively recruited by SARS-CoV-2 genome to alter transcriptional and post-transcriptional regulation of host cells and to promote viral replication. |
Fecha de creación:
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29-10-2022 |
Derechos:
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open access
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https://creativecommons.org/licenses/by-nc/4.0/ |
Tipo de documento:
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Article |
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Uri:
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https://ddd.uab.cat/record/238605
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