Investigation of the behavior of various calcium phosphate material during in vitro cell culture

Abstract

The great acceptance of calcium phosphate materials in the biomedical field comes from their close similirity to the mineral phase of bone. This make CaPs excellent subtrates for bone regeneration applications. In spite of possessing excellent bioactivity, osteoconductivity and biocompatibility in vivo, their behaviour in vitro is not always satisfactory. This is the case of calcium phosphate cements (CPC) which show hampered cell proliferation and differentiation it is believed that is caused by the extrememly high sp3ecific surface of these materials the problem is not that simple as the high surface areas lead to high ionic exchanges thus complicating interpretation of results. Thus, the purpose of this project is to determines which factor has major implications in cell behaviour wether topography will be evalutated: altaTCP, betaTCP, CDHA coarse, CDHA fine, HA and DCPA. Characterization of the materials: X-ray diffraction, SEM and B.E.T. With regards and differentiation will be evaluated after 4 hours, 3 and 7 days by LDH and ALP measurements. In addition, the pH and ion content will also be measured.

Incoming