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Title:
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Fast quantitative method for the analysis of carotenoids in transgenic maize
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Author:
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Rivera Vélez, Sol Maiam; Vilaró, Francisca; Zhu, Changfu; Bai, Chao; Farré Martinez, Gemma; Christou, Paul; Canela i Garayoa, Ramon
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Notes:
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A fast method was developed to determine carotenoid content in transgenic maize seeds. The analysis was carried out using an ultrahigh-pressure liquid chromatograph coupled to a photodiode array detector and a mass spectrometer (UHPLC-PDA-MS/MS). Sixteen carotenoid pigments were detected and quantified in <13 min. In addition, it was possible to obtain good resolution of both polar xanthophylls and nonpolar carotenes. The method exhibited (a) a high degree of repeatability (%RSD < 13%), (b) linear calibration curves (R2 > 0.9952), (c) satisfactory recoveries for most of the pigments (between 82 and 108%), and (d) low detection (from 0.02 to 0.07 μg/mL) and quantification limits (from 0.05 to 0.20 μg/mL) (LOD and LOQ, respectively). The methodology was applied to the analysis of transgenic maize lines TM1, TM2, and TM3, expressing several carotenogenic genes.
We thank the Comissionat per a Universitats i Recerca del
DIUE de la Generalitat de Catalunya (Barcelona, Spain) and
the European Social Fund (ESF) for a Ph.D. fellowship to
S.M.R. This work was supported by the University of Lleida,
Ministry of Science and Innovation (MICINN), Spain
(BFU200761413 and CTQ2009-14699-C02-01), and an ERC
Advanced Grant (BIOFORCE) to P.C. |
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Subject(s):
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-Carotenoids
-Maize
-Ultrahigh-pressure liquid chromatography (UHPLC) |
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Rights:
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(c) American Chemical Society, 2013
info:eu-repo/semantics/restrictedAccess |
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Document type:
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article
publishedVersion |
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Published by:
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American Chemical Society
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