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Title:
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Multiple Resistance to Synthetic Auxin Herbicides and Glyphosate in Parthenium hysterophorus Occurring in Citrus Orchards
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Author:
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Mora, Andrés D.; Rosario, Jesús; Rojano-Delgado, Antonia M.; Palma-Bautista, Candelario; Torra Farré, Joel; Alcántara-de la Cruz, Ricardo; Prado, Rafael de
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Notes:
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Dominican farmers have started to apply synthetic auxin herbicides (SAHs) as the main alternative to mitigate the impacts of the occurrence of glyphosate-resistant (GR) Parthenium hysterophorus populations in citrus orchards. A GR P. hysterophorus population survived field labeled rates of glyphosate, 2,4-dichlorophenoxyacetic acid (2,4-D), dicamba, and picloram, which showed poor control (<50%). In in vivo assays, resistance levels were high for glyphosate and moderate for picloram, dicamba, and 2,4-D. Sequencing the 5-enolpyruvylshikimate-3-phosphate synthase gene revealed the double Thr-102-Ile and Pro-106-Ser amino acid substitution, conferring resistance to glyphosate. Additionally, reduced absorption and impaired translocation contributed to this resistance. Regarding SAH, impaired 2,4-D transport and enhanced metabolism were confirmed in resistant plants. The application of malathion improved the efficacy of SAHs (control >50%), showing that metabolism of these herbicides was mediated by cytochrome P450 enzymes. This study reports, for the first time, multiple resistance to SAHs and glyphosate in P. hysterophorus.
This work was funded by the AGL2016-78944-R project (MINECO, Spain). |
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Subject(s):
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-Cytochrome P450 monooxigenase
-Enhanced metabolism
-EPSPS
-Impaired translocation
-Ragweed parthenium
-Reduced absorption
-TIPS mutation |
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Rights:
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(c) American Chemical Society, 2019
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Document type:
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Article
Article - Accepted version |
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Published by:
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American Chemical Society
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