dc.contributor.author |
Mora, Andrés D. |
dc.contributor.author |
Rosario, Jesús |
dc.contributor.author |
Rojano-Delgado, Antonia M. |
dc.contributor.author |
Palma-Bautista, Candelario |
dc.contributor.author |
Torra Farré, Joel |
dc.contributor.author |
Alcántara-de la Cruz, Ricardo |
dc.contributor.author |
Prado, Rafael de |
dc.date |
2019-09-13T15:43:32Z |
dc.date |
2020-08-15T22:21:17Z |
dc.date |
2019-08-15 |
dc.date |
2019-09-13T15:43:32Z |
dc.identifier |
0021-8561 |
dc.identifier |
http://hdl.handle.net/10459.1/66698 |
dc.identifier |
https://doi.org/10.1021/acs.jafc.9b03988 |
dc.identifier.uri |
http://hdl.handle.net/10459.1/66698 |
dc.description |
Dominican farmers have started to apply synthetic auxin herbicides (SAHs) as the main alternative to mitigate the impacts of the occurrence of glyphosate-resistant (GR) Parthenium hysterophorus populations in citrus orchards. A GR P. hysterophorus population survived field labeled rates of glyphosate, 2,4-dichlorophenoxyacetic acid (2,4-D), dicamba, and picloram, which showed poor control (<50%). In in vivo assays, resistance levels were high for glyphosate and moderate for picloram, dicamba, and 2,4-D. Sequencing the 5-enolpyruvylshikimate-3-phosphate synthase gene revealed the double Thr-102-Ile and Pro-106-Ser amino acid substitution, conferring resistance to glyphosate. Additionally, reduced absorption and impaired translocation contributed to this resistance. Regarding SAH, impaired 2,4-D transport and enhanced metabolism were confirmed in resistant plants. The application of malathion improved the efficacy of SAHs (control >50%), showing that metabolism of these herbicides was mediated by cytochrome P450 enzymes. This study reports, for the first time, multiple resistance to SAHs and glyphosate in P. hysterophorus. |
dc.description |
This work was funded by the AGL2016-78944-R project (MINECO, Spain). |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
American Chemical Society |
dc.relation |
MINECO/PN2013-2016/AGL2016-78944-R |
dc.relation |
Versió postprint del document publicat a: https://doi.org/10.1021/acs.jafc.9b03988 |
dc.relation |
Journal of Agricultural and Food Chemistry, 2019, vol. 67, num. 36, p. 10010-10017 |
dc.rights |
(c) American Chemical Society, 2019 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Cytochrome P450 monooxigenase |
dc.subject |
Enhanced metabolism |
dc.subject |
EPSPS |
dc.subject |
Impaired translocation |
dc.subject |
Ragweed parthenium |
dc.subject |
Reduced absorption |
dc.subject |
TIPS mutation |
dc.title |
Multiple Resistance to Synthetic Auxin Herbicides and Glyphosate in Parthenium hysterophorus Occurring in Citrus Orchards |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/acceptedVersion |