Título:
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Acinetobacter dijkshoorniae sp. nov., a new member of the
Acinetobacter calcoaceticus-Acinetobacter baumannii complex
mainly recovered from clinical samples in different countries
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Autor/a:
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Cosgaya, Clara; Marí Almirall, Marta; Van Assche, Ado; Fernández-Orth, Dietmar; Mosqueda, Noraida; Telli, Murat; Huys, Geert; Higgins, Paul G.; Seifert, Harald; Lievens, Bart; Roca, Ignasi; Vila Estapé, Jordi
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Abstract:
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The recent advances in bacterial species identification methods
have led to the rapid taxonomic diversification of the genus
Acinetobacter. In the present study, phenotypic and molecular
methods have been used to determine the taxonomic position of a
group of 12 genotypically distinct strains belonging to the
Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB)
complex, initially described by Gerner-Smidt and Tjernberg in
1993, that are closely related to A. pittii. Strains
characterized in this study originated mostly from human samples
obtained in different countries over a period of 15 years. rpoB
and MLST sequences were compared against those of 94 strains
representing all species included in the ACB complex. Cluster
analysis based on such sequences showed that all 12 strains
grouped together in a distinct clade closest to A. pittii that
was supported by bootstrap values of 99%. Values of average
nucleotide identity based on BLAST between the genome sequence
of strain JVAP01 (NCBI accession n masculine. LJPG00000000) and
those of other species from the ACB were always < 91.2%,
supporting the species status of the group. In addition, the
metabolic characteristics of the group matched those of the ACB
complex and the analysis of their protein signatures by
matrix-assisted laser desorption ionization-time-of-flight mass
spectrometry identified some specific peaks. Our results support
the designation of these strains as a novel species and we
propose the name A. dijkshoorniae sp. nov. The type strain is
JVAP01T (CECT 9134T, LMG 29605T). |
Materia(s):
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-Bacteriologia -Genètica bacteriana -Bacteriology -Bacterial genetics |
Derechos:
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(c) Microbiology Society, 2016
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Tipo de documento:
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Artículo Artículo - Versión aceptada |
Editor:
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Microbiology Society
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Compartir:
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