Título:
|
Succinyl-CoA Synthetase: New Antigen Candidate of Bartonella
bacilliformis
|
Autor/a:
|
Gomes, Cláudia; Palma, Noemí; Pons, Maria J.; Magallón Tejada, Ariel; Sandoval, Isabel; Tinco-Valdez, Carmen; Gutarra, Carlos; Valle Mendoza, Juana del; Ruiz, Joaquim; Matsuoka, Mayumi
|
Abstract:
|
BACKGROUND: Bartonella bacilliformis is the causative agent of
Carrion's disease, a neglected illness with mortality rates of
40-85% in the absence of treatment. The lack of a diagnostic
technique to overcome misdiagnosis and treat asymptomatic
carriers is of note. This study aimed to identify new B.
bacilliformis antigenic candidates that could lead to a new
diagnostic tool able to be implemented in endemic rural areas.
METHODOLOGY/PRINCIPAL FINDINGS: Blood (n = 198) and serum (n =
177) samples were collected in northern Peru. Clinical data were
recorded. Specific 16S rRNA amplification by RT-PCR, IFA and
ELISA for IgM/IgG with whole cells as antigens was done. Western
blot analysis and N-terminal amino acid sequencing detected
seroreactive proteins. ELISAs for IgM/IgG for the antigenic
candidates were performed. Of the population 33.3% reported at
least one symptom compatible with Carrion's disease; 25.4%
(IFA), 27.1% (ELISA-IgG), 33.9% (ELISA-IgM) and 38.9% (RT-PCR)
of samples were positive. Four proteins were considered
potential antigenic candidates, including two new antigenic
candidates, succinyl-CoA synthetase subunit alpha (SCS-alpha)
and succinyl-CoA synthetase subunit beta (SCS-beta). On Western
blot both Pap31 and SCS-alpha interacted with IgM, while GroEL
and SCS-beta interacted with IgG. The presence of specific
antibodies against the antigenic candidates varied from 34.5%
(IgG against SCS-alpha) to 97.2% (IgM against Pap31).
CONCLUSIONS/SIGNIFICANCE: RT-PCR and the high levels of
positivity for specific ELISAs demonstrate high levels of B.
bacilliformis exposure and asymptomatic carriers among
inhabitants. The new antigens identified might be used as a new
rapid diagnostic tool to diagnose acute Carrion's disease and
identify asymptomatic carriers. |
Materia(s):
|
-Malalties bacterianes -Paisatge rural -Antígens bacterians -Bacterial diseases -Rural landscape -Bacterial antigens |
Derechos:
|
cc by (c) Gomes et al., 2016
http://creativecommons.org/licenses/by/3.0/es/ |
Tipo de documento:
|
Artículo Artículo - Versión publicada |
Editor:
|
Public Library of Science (PLoS)
|
Compartir:
|
|