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Título:	Gene expression changes in blood RNA after swimming in a chlorinated pool
Autor/a:	Salas, Lucas A.; Font Ribera, Laia; Bustamante Pineda, Mariona; Sumoy Van Dyck, Lauro; Grimalt Obrador, Joan; Bonnin, Sarah; Aguilar Morón, María A.; Mattlin, Heidi; Hummel, Manuela; Ferrer Del Alamo, Ana; Kogevinas, Manolis; Villanueva Belmonte, Cristina
Abstract:	Exposure to disinfection by-products (DBP) such as trihalomethanes (THM) in swimming pools has been linked to adverse health effects in humans, but their biological mechanisms are unclear. We evaluated short-term changes in blood gene expression of adult recreational swimmers after swimming in a chlorinated pool. Volunteers swam 40min in an indoor chlorinated pool. Blood samples were drawn and four THM (chloroform, bromodichloromethane, dibromochloromethane and bromoform) were measured in exhaled breath before and after swimming. Intensity of physical activity was measured as metabolic equivalents (METs). Gene expression in whole blood mRNA was evaluated using IlluminaHumanHT-12v3 Expression-BeadChip. Linear mixed models were used to evaluate the relationship between gene expression changes and THM exposure. Thirty-seven before-after pairs were analyzed. The median increase from baseline to after swimming were: 0.7 to 2.3 for MET, and 1.4 to 7.1μg/m3 for exhaled total THM (sum of the four THM). Exhaled THM increased on average 0.94μg/m3 per 1 MET. While 1643 probes were differentially expressed post-exposure. Of them, 189 were also associated with exhaled levels of individual/total THM or MET after False Discovery Rate. The observed associations with the exhaled THM were low to moderate (Log-fold change range: -0.17 to 0.15). In conclusion, we identified short-term gene expression changes associated with swimming in a pool that were minor in magnitude and their biological meaning was unspecific. The high collinearity between exhaled THM levels and intensity of physical activity precluded mutually adjusted models with both covariates. These exploratory results should be validated in future studies.
Abstract:	This work was funded by the projects SAF2005-07643-C03-01/02/03 and SAF2007-62719 by Spanish Health Ministrygrants, and by FIS CP06/00341, FI06/00651 and CP01/3058 from the Fondo de Investigación Sanitaria (FIS), Ministerio de Sanidad; from the Plan Nacional, Ministerio de Educación y Ciencia. C.M.V. was supported by the Instituto de Salud Carlos III (CP06/00341); L.F.-R. was supported by a predoctoral fellowship (FI06/00651) from the Spanish Health Ministry; L.A.S. was supported by a Colciencias International PhD Scholarship (Grant: 529/2011), from the Fund for science and technology of Colombian Ministry of Education
Materia(s):	-Desinfecció i desinfectants -- Toxicologia -Contaminació -Expressió gènica -Piscines -Aigua -- Contaminació
Derechos:	info:eu-repo/semantics/embargoedAccess © Elsevier http://dx.doi.org/10.1016/j.jes.2017.05.011
Tipo de documento:	Artículo Artículo - Versión aceptada
Editor:	Elsevier
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