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Comparison of the immunoperoxidase monolayer assay and three commercial ELISAs for detection of antibodies against porcine circovirus type 2

To access the full text documents, please follow this link: http://hdl.handle.net/10459.1/66483

Author

Pileri, Emanuela

Cortey, Martí

Rodríguez, Fernando

Sibila, Marina

Fraile Sauce, Lorenzo José

Segalés, Joaquim

Publication date

2019-06-20T17:35:41Z

2019-06-20T17:35:41Z

2014

2019-06-20T17:35:41Z



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Abstract

The aim of this study was to compare and correlate antibody titres against porcine circovirus type 2 (PCV2) in porcine sera (n = 1270) obtained by immunoperoxidase monolayer assay (IPMA) with the results of three commercial ELISAs (designated E1, E2 and E3). The correlation between IPMA and ELISA results was excellent (r2 ≥ 0.90). Compared to IPMA, E2 had the highest sensitivity (93.0%), followed by E3 (90.1%) and E1 (85.0%); the specificity was 100% for all tests. All three commercial ELISAs had predictive values similar to those of IPMA and could be used to monitor antibody responses against PCV2 infection and/or vaccination.

Document Type

Article
Accepted version

Language

English

Subjects and keywords

Porcine circovirus type 2; ELISA; Serology; Immunoperoxidase monolayer assay

Publisher

Elsevier

Related items

Versió postprint del document publicat a: https://doi.org/10.1016/j.tvjl.2014.05.025

Veterinary Journal, 2014, vol. 201, num. 3, p. 429-432

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Rights

cc-by-nc-nd, (c) Elsevier, 2014

http://creativecommons.org/licenses/by-nc-nd/4.0/

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